metA:Gene: Difference between revisions

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!align=left {{table_heading_style}} |Standard name
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''metA''  
''metA''  
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!align=left {{table_heading_style}} |Mnemonic
!align=left |Mnemonic
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!align=left {{table_heading_style}} |Synonyms
!align=left |Synonyms
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''ECK4005, b4013, JW3973''<ref name='LIB:Riley_2006'/>
''ECK4005, b4013, JW3973''<ref name='LIB:Riley_2006'/>


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!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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*[http://biocyc.org/ECOLI/sequence?object=EG10581 EcoCyc Sequence Dump]  
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[[REL606]]
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NC_012967: 4193725..4194654<br />{{GBrowseFigureByCoord|4193725|4194654|REL606|NC_012967}}
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{{GBrowseFastaLink|ecoli_DH10B|NC_010473|4310141|4314786|FASTA Dump}}  
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NC_012967: 4193725..4194654<br />{{GBrowseFigureByCoord|4193725|4194654|REL606|NC_012967}}
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!|Feature type!!Location!!Description
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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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est. P1 cotransduction: 33% <ref name='LIB:Nichols_linked_Tn'/>  
est. P1 cotransduction: 33% <ref name='LIB:Nichols_linked_Tn'/>  


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Revision as of 02:08, 9 January 2010

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

metA

Mnemonic
Synonyms

ECK4005, b4013, JW3973[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

90.79 minutes, 90.79 minutes 

MG1655: 4212303..4213232
gbrowse_img.php?coord=4212303..4213232&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 4193725..4194654
gbrowse_img.php?coord=4193725..4194654&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 4217870..4218799
gbrowse_img.php?coord=4217870..4218799&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 4311999..4312928
gbrowse_img.php?coord=4311999..4312928&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔmetA (Keio:JW3973)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10856[3]

metA28

CGSC:7080

metA115

CGSC:8439

metA160

CGSC:10344

ΔmetA780::kan

PMID:16738554[2]

CGSC:101824


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3973

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCCCGATTCGTGTGCCGGACGA

Primer 2:CCATCCAGCGTTGGATTCATGTG

thiC39::Tn10

Linked marker

CAG18500 = CGSC7472[3]

est. P1 cotransduction: 52% [5]
Synonyms:thi-39::Tn10

malF3089::Tn10

Linked marker

CAG12164 = CGSC7476[3]

est. P1 cotransduction: 33% [5]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10581

EchoBASE (EcoliWiki Page)

EchoBASE:EB0576

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b4013

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4970

EcoGene (EcoliWiki Page)

EcoGene:EG10581

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:G7E-0

CGSC (EcoliWiki Page)

CGSC:516

ASAP (EcoliWikiPage)

ASAP:ABE-0013121


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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