galE:Gene Product(s): Difference between revisions

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Revision as of 18:38, 8 October 2009

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

GalE

Synonyms

UDP-galactose-4-epimerase[1], B0759[2][1], GalD[2][1], GalE[2][1]

Product description

UDP-glucose 4-epimerase[2][3];

Component of UDP-glucose 4-epimerase[2][3]

UDP-galactose 4-epimerase; hexose-1-phosphate uridylyltransferase[4]

EC number (for enzymes)
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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference(s) Evidence Code with/from Aspect Notes Status
GO:0003824

catalytic activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001509

F

Seeded from EcoCyc 12.5 [5]

complete

GO:0003978

UDP-glucose 4-epimerase activity

GO_REF:0000002
GO_REF:0000003

IEA: Inferred from Electronic Annotation

InterPro:IPR005886
EC:5.1.3.2

F

Seeded from EcoCyc [6]

complete

GO:0005975

carbohydrate metabolic process

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0119

P

Seeded from EcoCyc [6]

complete

GO:0006012

galactose metabolic process

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR005886
SP_KW:KW-0299

P

Seeded from EcoCyc [6]

complete

GO:0016853

isomerase activity

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0413

F

Seeded from EcoCyc [6]

complete

GO:0044237

cellular metabolic process

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001509

P

Seeded from EcoCyc [6]

complete

GO:0050662

coenzyme binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001509

F

Seeded from EcoCyc [6]

complete

GO:0005737

cytoplasm

C

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0005488

binding

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

F

Seeded from EcoCyc [6]

complete

GO:0005829

cytosol

PMID:16858726[7]

IDA: Inferred from Direct Assay

C

Seeded from EcoCyc [6]

complete

GO:0008152

metabolic process

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR016040

P

Seeded from EcoCyc [6]

complete

GO:0009242

colanic acid biosynthetic process

PMID:6090411[8]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc 11.1[3].

complete

GO:0016052

carbohydrate catabolic process

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0009435

NAD biosynthetic process

P

Seeded from EcoCyc 11.1[3].

required fields missing

Under review

GO:0009269

response to desiccation

P

Seeded from EcoCyc 11.1[3].

See discussion page.

required field missing

Under review

GO:0042603

capsule

C

Seeded from EcoCyc 11.1[3].

GalE is not located in the capsule.

required field missing

GO:0006012

galactose metabolic process

PMID:7025059[9]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0005975

carbohydrate metabolic process

PMID:7025059[9]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0006012

galactose metabolic process

PMID:13914787[10]

IMP: Inferred from Mutant Phenotype

P

Seeded from EcoCyc 12.5 [5]

complete

GO:0016052

carbohydrate catabolic process

PMID:7025059[9]

IGI: Inferred from Genetic Interaction

P

complete

GO:0016857

racemase and epimerase activity, acting on carbohydrates and derivatives

PMID:1821806[11]

IDA: Inferred from Direct Assay

F

complete

GO:0006012

galactose metabolic process

PMID:1821806[11]

IDA: Inferred from Direct Assay

P

complete

GO:0005737

cytoplasm

PMID:1821806[11]

IDA: Inferred from Direct Assay

C

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of UDP-glucose 4-epimerase

could be indirect

Protein

rsuA

PMID:16606699[12]

Experiment(s):EBI-1137880

Protein

yfcH

PMID:16606699[12]

Experiment(s):EBI-1137880

Protein

nadE

PMID:16606699[12]

Experiment(s):EBI-1137880

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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Source Notes
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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MRVLVTGGSG YIGSHTCVQL LQNGHDVIIL DNLCNSKRSV LPVIERLGGK HPTFVEGDIR
NEALMTEILH DHAIDTVIHF AGLKAVGESV QKPLEYYDNN VNGTLRLISA MRAANVKNFI
FSSSATVYGD QPKIPYVESF PTGTPQSPYG KSKLMVEQIL TDLQKAQPDW SIALLRYFNP
VGAHPSGDMG EDPQGIPNNL MPYIAQVAVG RRDSLAIFGN DYPTEDGTGV RDYIHVMDLA
DGHVVAMEKL ANKPGVHIYN LGAGVGNSVL DVVNAFSKAC GKPVNYHFAP RREGDLPAYW
ADASKADREL NWRVTRTLDE MAQDTWHWQS RHPQGYPD
Length

338

Mol. Wt

37.264 kDa

pI

6.3 (calculated)

Extinction coefficient

48,360 - 48,735 (calc based on 14 Y, 5 W, and 3 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes
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<motif_map/>

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=galE taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:UDPGLUCEPIM-MONOMER

UniProt (EcoliWiki Page)

UniProt:P09147

PFAM (EcoliWiki Page)

PFAM:PF01370

RefSeq (EcoliWiki Page)

RefSeq:NP_415280


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Notes

Links

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Name URL Comments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 1.4 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 3.4 3.5 3.6 3.7 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  6. ↑ 6.0 6.1 6.2 6.3 6.4 6.5 6.6 6.7 6.8 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  7. ↑ Lasserre, JP et al. (2006) A complexomic study of Escherichia coli using two-dimensional blue native/SDS polyacrylamide gel electrophoresis. Electrophoresis 27 3306-21 PubMed
  8. ↑ Trisler, P & Gottesman, S (1984) lon transcriptional regulation of genes necessary for capsular polysaccharide synthesis in Escherichia coli K-12. J. Bacteriol. 160 184-91 PubMed
  9. ↑ 9.0 9.1 9.2 Fietta, A et al. (1981) R-factor-mediated suppression of the galactose-sensitive phenotype of Escherichia coli K-12 galE Mutants. Plasmid 6 78-85 PubMed
  10. ↑ SOFFER, RL (1961) Enzymatic expression of genetic units of function concerned with galactose metabolism in Escherichia coli. J. Bacteriol. 82 471-8 PubMed
  11. ↑ 11.0 11.1 11.2 Vorgias, CE et al. () Overexpression and purification of the galactose operon enzymes from Escherichia coli. Protein Expr. Purif. 2 330-8 PubMed
  12. ↑ 12.0 12.1 12.2 Arifuzzaman, M et al. (2006) Large-scale identification of protein-protein interaction of Escherichia coli K-12. Genome Res. 16 686-91 PubMed

Categories

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