arsB:Gene Product(s): Difference between revisions

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Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
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complete
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GO:0008490
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arsenite secondary active transmembrane transporter activity
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PMID:14970228
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IDA: Inferred from Direct Assay
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F
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Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
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complete  
complete  
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GO:0008490
GO:0015105
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arsenite secondary active transmembrane transporter activity
arsenite transmembrane transporter activity
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PMID:14970228
GO_REF:0000002
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IDA: Inferred from Direct Assay
IEA: Inferred from Electronic Annotation
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InterPro:IPR000802
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F
F

Revision as of 16:46, 11 March 2009

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

ArsB

Synonyms

arsenite/antimonite transporter[1], B3502[2][1], ArsF[2][1], F172[2][1]

Product description

ArsB[2][3];

Component of arsenate Ars transporter[2][3]

Arsenite pump, inner membrane; resistance to arsenate, arsenite, and antimonite[4]

EC number (for enzymes)


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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference(s) Evidence Code with/from Aspect Notes Status
GO:0016020

membrane

GO_REF:0000004
GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0397
SP_KW:KW-0472

C

Seeded from EcoCyc 12.5 [5]

complete

GO:0016021

integral to membrane

GO_REF:0000002
GO_REF:0000004

IEA: Inferred from Electronic Annotation

InterPro:IPR000802
SP_KW:KW-0812

C

Seeded from EcoCyc 12.5 [5]

complete

GO:0008490

arsenite secondary active transmembrane transporter activity

PMID:14970228[6]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc [7]

complete

GO:0015699

antimonite transport

PMID:14970228[6]

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc 12.5 [5]

complete

GO:0015700

arsenite transport

PMID:14970228[6]

IDA: Inferred from Direct Assay

P

Seeded from EcoCyc 12.5 [5]

complete

GO:0042960

antimonite secondary active transmembrane transporter activity

PMID:14970228[6]

IDA: Inferred from Direct Assay

F

Seeded from EcoCyc 12.5 [5]

complete

GO:0046685

response to arsenic

PMID:7860609[8]

IEP: Inferred from Expression Pattern

P

Seeded from EcoCyc 12.5 [5]

complete

GO:0005886

plasma membrane

GO_REF:0000004
GO_REF:0000004
GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_KW:KW-0997
SP_KW:KW-1003
SP_SL:SL-0037

C

Seeded from EcoCyc [7]

complete

GO:0006810

transport

GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0813

P

Seeded from EcoCyc [7]

complete

GO:0006805

xenobiotic metabolic process

P

Seeded from EcoCyc 11.1[3].

required fields missing

GO:0015105

arsenite transmembrane transporter activity

GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR000802

F

Seeded from EcoCyc [7]

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of arsenate Ars transporter

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Notes

plasma membrane

C-terminus localized to the periplasm with 11 predicted transmembrane domains

Daley et al. (2005) [9]

plasma membrane

From EcoCyc[3]

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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MFYPGGIMLL AGAIFVLTIV LVIWQPKGLG IGWSATLGAV LALVTGVVHP GDIPVVWNIV
WNATAAFIAV IIISLLLDES GFFEWAALHV SRWGNGRGRL LFTWIVLLGA AVAALFANDG
AALILTPIVI AMLLALGFSK GTTLAFVMAA GFIADTASLP LIVSNLVNIV SADFFGLGFR
EYASVMVPVD IAAIVATLVM LHLYFRKDIP QNYDMALLKS PAEAIKDPAT FKTGWVVLLL
LLVGFFVLEP LGIPVSAIAA VGALILFVVA KRGHAINTGK VLRGAPWQIV IFSLGMYLVV
YGLRNAGLTE YLSGVLNVLA DNGLWAATLG TGFLTAFLSS IMNNMPTVLV GALSIDGSTA
SGVIKEAMVY ANVIGCDLGP KITPIGSLAT LLWLHVLSQK NMTISWGYYF RTGIIMTLPV
LFVTLAALAL RLSFTL
Mol. Wt

45.5 kDa (calc) [2]

pI
Extinction coefficient

80900 - 81025 (calc based on 10 Y, 12 W, and 1 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes

Domain

412 - 434

Predicted membrane-spanning helix.

TMD predicted by TMHMM[10]

Domain

284 - 303

Predicted membrane-spanning helix.

TMD predicted by TMHMM[10]

Domain

315 - 337

Predicted membrane-spanning helix.

TMD predicted by TMHMM[10]

Domain

182 - 204

Predicted membrane-spanning helix.

TMD predicted by TMHMM[10]

Domain

235 - 269

Predicted membrane-spanning helix.

TMD predicted by TMHMM[10]

Domain

98 - 117

Predicted membrane-spanning helix.

TMD predicted by TMHMM[10]

Domain

121 - 138

Predicted membrane-spanning helix.

TMD predicted by TMHMM[10]

Domain

145 - 167

Predicted membrane-spanning helix.

TMD predicted by TMHMM[10]

Domain

29 - 51

Predicted membrane-spanning helix.

TMD predicted by TMHMM[10]

Domain

55 - 77

Predicted membrane-spanning helix.

TMD predicted by TMHMM[10]

Domain

5 - 24

Predicted membrane-spanning helix.

TMD predicted by TMHMM[10]

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tmhmm.php?gene=arsB&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure>arsB 562</beststructure>

Models

View models at:

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:ARSF-MONOMER

UniProt (EcoliWiki Page)

UniProt:P0AB93

PFAM (EcoliWiki Page)

PFAM:PF02040

RefSeq (EcoliWiki Page)

RefSeq:NP_417959


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Notes

Links

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Name URL Comments
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References

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  1. ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 2.5 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ 5.0 5.1 5.2 5.3 5.4 5.5 EcoCyc (release 12.5; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  6. ↑ 6.0 6.1 6.2 6.3 Meng, YL et al. (2004) As(III) and Sb(III) uptake by GlpF and efflux by ArsB in Escherichia coli. J. Biol. Chem. 279 18334-41 PubMed
  7. ↑ 7.0 7.1 7.2 7.3 EcoCyc (release 13.0; 2009) Keseler, IM et al. (2009) Nucleic Acids Res. 37(Database issue):D464-70
  8. ↑ Carlin, A et al. (1995) The ars operon of Escherichia coli confers arsenical and antimonial resistance. J. Bacteriol. 177 981-6 PubMed
  9. ↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed
  10. ↑ 10.00 10.01 10.02 10.03 10.04 10.05 10.06 10.07 10.08 10.09 10.10 Krogh, A et al. (2001) Predicting transmembrane protein topology with a hidden Markov model: application to complete genomes. J. Mol. Biol. 305 567-80 PubMed


Categories

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