pitA:Gene: Difference between revisions

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{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"
 
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!{{table_heading_style}} |Standard name
!align=left {{table_heading_style}} |Standard name
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''pitA''  
''pitA''  
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!{{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Mnemonic
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!{{table_heading_style}} |Synonyms
!align=left {{table_heading_style}} |Synonyms
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''ECK3478, b3493, JW3460, pit, G442''<ref name='LIB:Riley_2006'/><ref name='LIB:EcoCyc10.6'/>
''ECK3478, b3493, JW3460, pit, G442''<ref name='LIB:Riley_2006'/><ref name='LIB:EcoCyc10.6'/>
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===Notes===
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!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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MG1655
MG1655
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{{GBrowseFastaLink|ecoli|NC_000913|3635665|3637164|FASTA Dump}}
{{GBrowseFastaLink|ecoli|NC_000913|3635665|3637164|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG12230 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG12230 EcoCyc Sequence Dump]  
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W3110
W3110
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{{GBrowseFastaLink|ecoli_W3110|AC_000091|4001274|4002773|FASTA Dump}}  
{{GBrowseFastaLink|ecoli_W3110|AC_000091|4001274|4002773|FASTA Dump}}  
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===Notes===
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|[[PMID:16738554]]<ref name=PMID:16738554/>
[[PMID:16738554]]<ref name=PMID:16738554/>
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[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=108844 CGSC10563]<ref name='LIB:cgsc'/>  
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=108844 CGSC10563]<ref name='LIB:cgsc'/>  
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|[[PMID:4570598]]<ref name=PMID:4570598/>[[PMID:4568763]]<ref name=PMID:4568763/>
[[PMID:4570598]]<ref name=PMID:4570598/> [[PMID:4568763]]<ref name=PMID:4568763/>
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[[cgscmutation:5633|CGSC:5633]]
[[cgscmutation:5633|CGSC:5633]]
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|[[PMID:16738554]]<ref name=PMID:16738554/>
[[PMID:16738554]]<ref name=PMID:16738554/>
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[[cgscmutation:102374|CGSC:102374]]
[[cgscmutation:102374|CGSC:102374]]
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===Notes===
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JW3460
JW3460
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[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
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|[[PMID:16769691]]<ref name=PMID:16769691/>
[[PMID:16769691]]<ref name=PMID:16769691/>


Status:Clone OK
Status:Clone OK
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Primer 2:CCaAGGAACTGCAAGGAGAGCCA  
Primer 2:CCaAGGAACTGCAAGGAGAGCCA  
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zhg-50::Tn10
zhg-50::Tn10
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CAG18450 = [http://cgsc2.biology.yale.edu/Strain.php?ID=37863 CGSC7450]<ref name='LIB:cgsc'/>
CAG18450 = [http://cgsc2.biology.yale.edu/Strain.php?ID=37863 CGSC7450]<ref name='LIB:cgsc'/>
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est. P1 cotransduction: 14% <ref name='LIB:Nichols_linked_Tn'/>
est. P1 cotransduction: 14% <ref name='LIB:Nichols_linked_Tn'/>nnSynonyms:zhf-50::Tn10, zhf-5::Tn10  
 
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zic-4901::Tn10
zic-4901::Tn10
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est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/>  
est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/>  
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[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0011407&formSubmitReturn=1 ASAP:ABE-0011407]
[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0011407&formSubmitReturn=1 ASAP:ABE-0011407]
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<noinclude><protect>
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==References==
==References==
{{BacktoTop}}
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<references/>
<references/>
__NOTOC__
__NOTOC__

Revision as of 21:32, 21 January 2009

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

pitA

Mnemonic
Synonyms

ECK3478, b3493, JW3460, pit, G442[1][2]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

78.36 minutes 

MG1655: 3635665..3637164
gbrowse_img.php?coord=3634665..3638164&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 4002773..4001274
gbrowse_img.php?coord=4000274..4003773&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔpitA (Keio:JW3460)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC10563[4]

pitA10

PMID:4570598[5]PMID:4568763[6]

CGSC:5633

pitA1

CGSC:15787

ΔpitA749::kan

PMID:16738554[3]

CGSC:102374


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3460

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCCTACATTTGTTTGCTGGCCT

Primer 2:CCaAGGAACTGCAAGGAGAGCCA

zhg-50::Tn10

Linked marker

CAG18450 = CGSC7450[4]

est. P1 cotransduction: 14% [8]nnSynonyms:zhf-50::Tn10, zhf-5::Tn10

zic-4901::Tn10

Linked marker

CAG18492 = CGSC7457[4]

est. P1 cotransduction: % [8]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG12230

EchoBASE (EcoliWiki Page)

EchoBASE:EB2142

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3493

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4291

EcoGene (EcoliWiki Page)

EcoGene:EG12230

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:PITA

ASAP (EcoliWikiPage)

ASAP:ABE-0011407


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Notes

Links

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Name URL Comments
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References

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  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ Willsky, GR et al. (1973) Inorganic phosphate transport in Escherichia coli: involvement of two genes which play a role in alkaline phosphatase regulation. J. Bacteriol. 113 529-39 PubMed
  6. ↑ Bachmann, BJ (1972) Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev 36 525-57 PubMed
  7. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).


Categories

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