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!Database!!Accession!!Notes
!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])  
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])  
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11336 EcoCyc:EG11336]
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11336 EcoCyc:EG11336]
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[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])  
[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])  
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[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB1312 EchoBASE:EB1312]
[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB1312 EchoBASE:EB1312]
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[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])  
[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])  
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[http://chase.ou.edu/macro/searchlink.php?bnum=b2945 EcoliGenExpDB:b2945]
[http://chase.ou.edu/macro/searchlink.php?bnum=b2945 EcoliGenExpDB:b2945]
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[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])  
[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])  
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[http://BioCyc.org/ECOL199310/new-image?object=C3531 ECOL199310CYC:C3531]
[http://BioCyc.org/ECOL199310/new-image?object=C3531 ECOL199310CYC:C3531]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])  
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])  
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[http://ecogene.org/geneinfo.php?eg_id=EG11336 EcoGene:EG11336]
[http://ecogene.org/geneinfo.php?eg_id=EG11336 EcoGene:EG11336]
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[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])  
[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])  
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[http://BioCyc.org/ECOO157/new-image?object=ENDA ECOO157CYC:ENDA]
[http://BioCyc.org/ECOO157/new-image?object=ENDA ECOO157CYC:ENDA]
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[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])  
[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])  
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[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/824 CGSC:824]
[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/824 CGSC:824]
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[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0009661&formSubmitReturn=1 ASAP:ABE-0009661] 
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Revision as of 00:19, 16 September 2008

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

endA

Mnemonic
Synonyms

ECK2940, b2945, JW2912[1]

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

66.56 minutes 

MG1655: 3088369..3089076
gbrowse_img.php?coord=3087369..3090076&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 3089003..3089710
gbrowse_img.php?coord=3088003..3090710&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Notes

Sequence Features

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Feature type Location Description
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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Notes

Alleles and Phenotypes

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Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔendA (Keio:JW2912)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10253[3]

endA1

CGSC:5430

endA2

CGSC:5609

endA101

CGSC:8416

endA5

CGSC:10668

endA3

CGSC:14266

endA4

CGSC:16230

endA6(del-ins)::kan

CGSC:35763

endA7(del-ins)::Cm

CGSC:35768

endA8(del:Frt:Tc)::Tc

PMID:7789817[4]

CGSC:59095

endA9(del-ins)::FRT

CGSC:64683

ΔendA720::kan

PMID:16738554[2]

CGSC:100710


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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Notes

EndA is an enzyme in many proteobacteria. One function of the enzyme is cleaving unspecific internal sites at both RNA and DNA.[5] Cultures of mutant endA result in large amounts of chromosmal and plasmid DNA, which does not appear in the wild type allele. EndA is responsible for DNA and plasmid DNA decomposition [6].

Genetic Resources

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Resource Resource Type Source Notes/Reference

JW2912

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCTACCGTTATTTGTCTATTGC

Primer 2:CCGCTCTTTCGCGCCTGGCAAGC

speA210::Tn10

Linked marker

CAG12168 = CGSC7431[3]

est. P1 cotransduction: 91% [8]

Synonyms:zgd-210::Tn10, zgf-210::Tn10

nupG511::Tn10

Linked marker

CAG18472 = CGSC7433[3]

est. P1 cotransduction: 58% [8]

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Notes

Accessions in Other Databases

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Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11336

EchoBASE (EcoliWiki Page)

EchoBASE:EB1312

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2945

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3531

EcoGene (EcoliWiki Page)

EcoGene:EG11336

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:ENDA

CGSC (EcoliWiki Page)

CGSC:824

ASAP (EcoliWikiPage)

ASAP:ABE-0009661


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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Notes

Links

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Name URL Comments
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See Help:Links_table for how to enter or edit information in this section of EcoliWiki.

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Cherepanov, PP & Wackernagel, W (1995) Gene disruption in Escherichia coli: TcR and KmR cassettes with the option of Flp-catalyzed excision of the antibiotic-resistance determinant. Gene 158 9-14 PubMed
  5. ↑ Altermark, B et al. (2007) Comparative studies of endonuclease I from cold-adapted Vibrio salmonicida and mesophilic Vibrio cholerae. FEBS J. 274 252-63 PubMed
  6. ↑ Lin, JJ (1992) Endonuclease A degrades chromosomal and plasmid DNA of Escherichia coli present in most preparations of single stranded DNA from phagemids. Proc. Natl. Sci. Counc. Repub. China B 16 1-5 PubMed
  7. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).


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