recF:Gene: Difference between revisions

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[[cgscmutation:10318|CGSC:10318]]
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''recF400::Tn5''
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[[PMID:2841294]]<ref name=PMID:2841294/>
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[[cgscmutation:67402|CGSC:67402]]
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Revision as of 15:40, 21 August 2008

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

recF

Mnemonic
Synonyms

ECK3692, b3700, JW3677, uvrF[1]

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

83.59 minutes, 83.59 minutes 

MG1655: 3879244..3878171
gbrowse_img.php?coord=3877171..3880244&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 3759194..3760267
gbrowse_img.php?coord=3758194..3761267&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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Feature type Location Description
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Notes

Alleles and Phenotypes

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Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔrecF (Keio:JW3677)

deletion

deletion

PMID:16738554[2]

Shigen

recF143

CGSC:10318

recF400::Tn5

PMID:2841294[3]

CGSC:67402


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Notes

Genetic Resources

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Resource Resource Type Source Notes/Reference

JW3677

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCTCCCTCACCCGCTTGTTGAT

Primer 2:CCATCCGTTATTTTACCCTTTTC

zid-501::Tn10

Linked marker

CAG18499 = CGSC7458[5]

est. P1 cotransduction: 87% [6]

rbsD296::Tn10

Linked marker

CAG18501 = CGSC7460[5]

est. P1 cotransduction: 9% [6]

Synonyms:zie-296::Tn10

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Notes

Accessions in Other Databases

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Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10828

EchoBASE (EcoliWiki Page)

EchoBASE:EB0821

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3700

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4622

EcoGene (EcoliWiki Page)

EcoGene:EG10828

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:RECF

CGSC (EcoliWiki Page)

CGSC:308

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Notes

Links

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Name URL Comments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ Thoms, B & Wackernagel, W (1988) Suppression of the UV-sensitive phenotype of Escherichia coli recF mutants by recA(Srf) and recA(Tif) mutations requires recJ+. J. Bacteriol. 170 3675-81 PubMed
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).


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