carB:Gene: Difference between revisions
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!Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | !|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments | ||
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ΔcarB (Keio:JW0031) | ΔcarB (Keio:JW0031) | ||
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[[PMID:16738554]]<ref name=PMID:16738554/> | [[PMID:16738554]]<ref name=PMID:16738554/> | ||
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[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=106630 CGSC8349]<ref name='LIB:cgsc'/> | [http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=106630 CGSC8349]<ref name='LIB:cgsc'/> | ||
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carB96::Tn10 | carB96::Tn10 | ||
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Synonyms: car-96::Tn10 | Synonyms: car-96::Tn10 | ||
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''carB671'' | |||
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[[PMID:4373646]]<ref name=PMID:4373646/> | |||
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[[cgscmutation:5037|CGSC:5037]] | |||
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Revision as of 15:09, 21 August 2008
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Alleles and Phenotypes | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
| Standard name |
carB |
|---|---|
| Mnemonic | |
| Synonyms |
ECK0034, b0033, JW0031, arg+ura, cap, pyrA[1] |
| edit table | |
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Notes
Location(s) and DNA Sequence
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
0.66 minutes |
MG1655: 30817..34038 |
||
|
W3110 |
|
W3110: 30817..34038 |
||
| edit table |
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Notes
Sequence Features
| Feature type | Location | Description |
|---|---|---|
| edit table |
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Notes
Alleles and Phenotypes
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔcarB (Keio:JW0031) |
deletion |
deletion |
|||||
|
carB96::Tn10 |
Insertion at 33,963 bp in MG1655 (NC_000913) |
adapted from Nichols et al.[4] |
Synonyms: car-96::Tn10 | ||||
|
carB671 |
| ||||||
| edit table |
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Notes
Genetic Resources
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0031 |
Plasmid clone |
Status:Clone OK Primer 1:GCCCCAAAACGTACAGATATAAA Primer 2:CCTTTGATCTGTGCGTGCATTTC | |
|
Linked marker |
est. P1 cotransduction: 30% [4] Synonyms:thr-34::Tn10 | ||
|
leuO3051::Tn10 |
Linked marker |
est. P1 cotransduction: 8% [4] Synonyms:zab-3051::Tn10
| |
| edit table |
See Help:Gene_resources for help entering information into the Genetic Resources table.
Notes
Accessions in Other Databases
| Database | Accession | Notes |
|---|---|---|
| edit table |
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
See Help:Links_table for how to enter or edit information in this section of EcoliWiki.
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 3.2 3.3 CGSC: The Coli Genetics Stock Center
- ↑ 4.0 4.1 4.2 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).
- ↑ Mergeay, M et al. (1974) Physiology and genetics of carbamoylphosphate synthesis in Escherichia coli K12. Mol. Gen. Genet. 133 299-316 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed