recJ:Gene: Difference between revisions

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edited by STLovett via TableEdit
edited by STLovett via TableEdit
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similar to recJ284, null
similar to recJ284, null
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PMID=6317649
<ref name 'PMID=6317649'/>
PMID=6257642
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recJ513
recJ153
recJ154
recJ154
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Reduces expression of recJ gene, can be suppressed by mutations in initiation factor IF3 for translation
Reduces expression of recJ gene, can be suppressed by mutations in initiation factor IF3 for translation
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PMID=4587405
<ref name 'PMID=4587405'/>
PMID=6317649
<ref name 'PMID=6317649'/>
PMID=9352920
<ref name 'PMID=9352920'/>
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Revision as of 18:39, 26 June 2008

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

recJ

Mnemonic
Synonyms

ECK2887, b2892, JW2860[1]

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

65.4 minutes 

MG1655: 3036128..3034395
gbrowse_img.php?coord=3033395..3037128&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 3036762..3035029
gbrowse_img.php?coord=3034029..3037762&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Notes

Sequence Features

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Feature type Location Description
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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Notes

Alleles and Phenotypes

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Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔrecJ (Keio:JW2860)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10217[3]

recJ284::Tn10

Insertion of Tn10 (Tetracycline-resistance) at nucleotide 434 relative to GTG start codon

truncation

null phenotype, recombination deficient and UV-sensitive in recBC sbcA and recBC sbcBC backgrounds. Highly synergistic for UV-sensitivity, recombination deficiency and partial inviability with recBC.

[4]

CGSC

recJ77

G to A nucleotide 281

G to D amino acid 78

similar to recJ284, null

Cite error: Invalid <ref> tag; refs with no name must have content Cite error: Invalid <ref> tag; refs with no name must have content

recJ153 recJ154

GTG to GTA at start codon

None

Reduces expression of recJ gene, can be suppressed by mutations in initiation factor IF3 for translation

Cite error: Invalid <ref> tag; refs with no name must have content Cite error: Invalid <ref> tag; refs with no name must have content Cite error: Invalid <ref> tag; refs with no name must have content

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Notes

Genetic Resources

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Resource Resource Type Source Notes/Reference

JW2860

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAAACAACAGATACAACTTCG

Primer 2:CCAATTGGCCAGATATTGTCGAT

recD1901::Tn10

Linked marker

CAG12135 = CGSC7429[3]

est. P1 cotransduction: % [6]

speA210::Tn10

Linked marker

CAG12168 = CGSC7431[3]

est. P1 cotransduction: 9% [6]

Synonyms:zgd-210::Tn10, zgf-210::Tn10

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Notes

Accessions in Other Databases

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Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10830

EchoBASE (EcoliWiki Page)

EchoBASE:EB0823

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2892

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3472

EcoGene (EcoliWiki Page)

EcoGene:EG10830

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:RECJ

CGSC (EcoliWiki Page)

CGSC:17965

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Notes

Links

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Name URL Comments
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See Help:Links_table for how to enter or edit information in this section of EcoliWiki.

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Lovett, ST & Clark, AJ (1984) Genetic analysis of the recJ gene of Escherichia coli K-12. J. Bacteriol. 157 190-6 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).


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