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Lacks DNA specifying six DEAD-box motifs that are critical for helicase activity
Lacks DNA specifying six DEAD-box motifs that are critical for helicase activity
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[[PMID:9776741]]<ref name=PMID:9776741/>
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Revision as of 18:27, 8 November 2007

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

hsdR

Mnemonic
Synonyms

ECK4340, b4350, JW4313, Hs, hsp, hsr, rm[1]

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

98.74 minutes 

MG1655: 4584838..4581272
gbrowse_img.php?coord=4580272..4585838&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 4591495..4587929
gbrowse_img.php?coord=4586929..4592495&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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Feature type Location Description
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Notes

Alleles and Phenotypes

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Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔhsdR (Keio:JW4313)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC11083[3]

hsdRΔ4

deletion

deletion

Lacks DNA specifying six DEAD-box motifs that are critical for helicase activity

PMID:9776741[4]

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Notes

Genetic Resources

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Resource Resource Type Source Notes/Reference

JW4313

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCTTATGGGCgTTAAATATTTGGACAGGCCCG

Primer 2:CCGGCCAGCTCGTCCCAGATATA

zjf-920::Tn10

Linked marker

CAG12019 = CGSC7484[3]

est. P1 cotransduction: % [6]

Synonyms:zjh-901::Tn10, zjg-920::Tn10

mdoB202::Tn10

Linked marker

CAG18430 = CGSC7488[3]

est. P1 cotransduction: 66% [6]

Synonyms:zjj-202::Tn10, zji-202::Tn10

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Notes

Accessions in Other Databases

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Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10459

EchoBASE (EcoliWiki Page)

EchoBASE:EB0454

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b4350

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C5425

EcoGene (EcoliWiki Page)

EcoGene:EG10459

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:Z5948

CGSC (EcoliWiki Page)

CGSC:620

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Notes

Links

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Name URL Comments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Davies, GP et al. (1998) EcoKI with an amino acid substitution in any one of seven DEAD-box motifs has impaired ATPase and endonuclease activities. Nucleic Acids Res. 26 4828-36 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).


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