endA:Gene: Difference between revisions
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= | Cultures of mutant endA result in large amounts of chromosmal and plasmid Dna, which does not appear in the wild type allele. EndA is responsable for Dna and plasmid Dna decomposition. <ref name='PMID:1631242. [2]'/> | ||
==Genetic Resources== | ==Genetic Resources== | ||
Revision as of 19:28, 6 November 2007
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Alleles and Phenotypes | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
| Standard name |
endA |
|---|---|
| Mnemonic | |
| Synonyms |
ECK2940, b2945, JW2912[1] |
| edit table | |
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
Notes
Location(s) and DNA Sequence
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
66.56 minutes |
MG1655: 3088369..3089076 |
||
|
W3110 |
|
W3110: 3089003..3089710 |
||
| edit table |
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
Notes
Sequence Features
| Feature type | Location | Description |
|---|---|---|
| edit table |
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
Cultures of mutant endA result in large amounts of chromosmal and plasmid Dna, which does not appear in the wild type allele. EndA is responsable for Dna and plasmid Dna decomposition. [2]
Alleles and Phenotypes
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔendA (Keio:JW2912) |
deletion |
deletion |
|||||
| edit table |
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
Cultures of mutant endA result in large amounts of chromosmal and plasmid Dna, which does not appear in the wild type allele. EndA is responsable for Dna and plasmid Dna decomposition. [5]
Genetic Resources
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW2912 |
Plasmid clone |
Status:Clone OK Primer 1:GCCTACCGTTATTTGTCTATTGC Primer 2:CCGCTCTTTCGCGCCTGGCAAGC | |
|
Linked marker |
est. P1 cotransduction: 91% [7] Synonyms:zgd-210::Tn10, zgf-210::Tn10 | ||
|
Linked marker |
est. P1 cotransduction: 58% [7] | ||
| edit table |
See Help:Gene_resources for help entering information into the Genetic Resources table.
Notes
Accessions in Other Databases
| Database | Accession | Notes |
|---|---|---|
| edit table |
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
See Help:Links_table for how to enter or edit information in this section of EcoliWiki.
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Lin, JJ (1992) Endonuclease A degrades chromosomal and plasmid DNA of Escherichia coli present in most preparations of single stranded DNA from phagemids. Proc. Natl. Sci. Counc. Repub. China B 16 1-5 PubMed
- ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
- ↑ Lin, JJ (1992) Endonuclease A degrades chromosomal and plasmid DNA of Escherichia coli present in most preparations of single stranded DNA from phagemids. Proc Natl Sci Counc Repub China B 16 1-5 [2 PubMed]
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).