mreB:Expression: Difference between revisions

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*Medium:  median data from rich and minimal media
*Medium:  median data from rich and minimal media
*Temperature (°C):  37
*Temperature (°C):  37
*Growth Rate(min):   
*Doubling time (min): Growth Rate(min):   
*other:
*Growth phase: other:
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emPAI
emPAI
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*Medium:  AB glycerol medium supplemented with 0.025% casamino acids (slowly growing cells)
*Medium:  AB glycerol medium supplemented with 0.025% casamino acids (slowly growing cells)
*Temperature (°C):  30°C
*Temperature (°C):  30°C
*Growth Rate(min):   
*Doubling time (min): Growth Rate(min):   
*other:
*Growth phase: other:
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quantitative western
quantitative western
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*Medium:  rapidly growing cells
*Medium:  rapidly growing cells
*Temperature (°C):   
*Temperature (°C):   
*Growth Rate(min):   
*Doubling time (min): Growth Rate(min):   
*other:
*Growth phase: other:
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quantitative western
quantitative western
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*Medium:   
*Medium:   
*Temperature (°C):   
*Temperature (°C):   
*Growth Rate(min):  *
*Doubling time (min): Growth Rate(min):  *
*other:  *
*Growth phase: other:  *
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*Medium:   
*Medium:   
*Temperature (°C):   
*Temperature (°C):   
*Growth Rate(min):  *
*Doubling time (min): Growth Rate(min):  *
*other:  *
*Growth phase: other:  *
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*Medium:   
*Medium:   
*Temperature (°C):   
*Temperature (°C):   
*Growth Rate(min):  *
*Doubling time (min): Growth Rate(min):  *
*other:  *
*Growth phase: other:  *
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PMID:20671182  
PMID:20671182  
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Protein
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''E. coli'' K-12 [http://ecoliwiki.net/colipedia/index.php/Category:Strain:EMG2 EMG2]
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60
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molecules/cell
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*Medium: MOPS glucose minimal
*Temperature (°C): 37
*Doubling time (min):
*Growth phase: Exponential
*OD: OD600 = 0.2 to 0.25 
*other: aerated
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Quantitative protein sequencing
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Spot ID: Mll0-1
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PMID: 9298646


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Revision as of 18:17, 21 July 2015

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Overview Transcription Translation Turnover Experimental Links References Suggestions

Overview

This is a placeholder for a summary statement on how expression of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Cellular Levels

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See Help:Cellular levels

Molecule Organism or Strain Value Units Experimental Conditions Assay used Notes Reference(s)

Protein

E. coli K-12 MC4100

1.49E+03

molecules/cell

  • Medium: median data from rich and minimal media
  • Temperature (°C): 37
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

emPAI

PMID:18304323[1]

Protein

E. coli K-12 MC1000

17,000

molecules/cell

  • Medium: AB glycerol medium supplemented with 0.025% casamino acids (slowly growing cells)
  • Temperature (°C): 30°C
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

quantitative western

PMID:14517265[2]

Protein

E. coli K-12 MC1000

40,000

molecules/cell

  • Medium: rapidly growing cells
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min):
  • Growth phase: other:

quantitative western

PMID:14517265[2]

Protein

Ecoli K-12

210.659+/-2.217

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

Single Molecule Fluorescence

PMID:20671182[3]

mRNA

Ecoli K-12

0.25036+/-0.0169

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by FISH

PMID:20671182[3]

mRNA

Ecoli K-12

0.229626079

Molecules/cell

  • Medium:
  • Temperature (°C):
  • Doubling time (min): Growth Rate(min): *
  • Growth phase: other: *

by RNA_Seq

PMID:20671182[3]

Protein

E. coli K-12 EMG2

60

molecules/cell

  • Medium: MOPS glucose minimal
  • Temperature (°C): 37
  • Doubling time (min):
  • Growth phase: Exponential
  • OD: OD600 = 0.2 to 0.25
  • other: aerated

Quantitative protein sequencing

Spot ID: Mll0-1

PMID:9298646[4]

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Notes

Transcription and Transcriptional Regulation

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See Help:Expression_transcription for help entering or editing information in this section of EcoliWiki.

Transcription unit(s)

mreBCD-yhdE-rng

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Figure courtesy of RegulonDB


Notes

This is a placeholder for a summary statement about how transcription of this gene is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.


Identification of transcription start sites and different levels of mreB and mreBCD transcripts.[5]

Translation and Regulation of Translation

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<gbrowseImage> name=NC_000913:3399089..3399129 source=MG1655 flip=1 type=Gene+DNA_+Protein preset=Nterminus </gbrowseImage> This picture shows the sequence around the N-terminus.


Notes

This is a placeholder for a summary statement about how translation of this gene product is regulated. You can help EcoliWiki by becoming a user and writing/editing this statement.

Turnover and Regulation of Turnover

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Notes

This is a placeholder for a summary statement about turnover of this gene product. You can help EcoliWiki by becoming a user and writing/editing this statement.

Experimental

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Mutations Affecting Expression See Help:Expression_mutations for help entering or editing information in this section of EcoliWiki.

Allele Name Mutation Phenotype Reference
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Expression Studies See Help:Expression_studies for help entering or editing information in this section of EcoliWiki.

Type Reference Notes

microarray

GEO Profiles:b3251 (EcoliWiki Page)

NCBI GEO profiles for mreB

microarray

GenExpDB:b3251 (EcoliWiki Page)

Summary of data for mreB from multiple microarray studies

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Expression Resources See Help:Expression_resources for help entering or editing information in this section of EcoliWiki.

Resource Name Resource Type Description Source Notes
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<protect>

Notes

Accessions Related to mreB Expression

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10608

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0603

Escherichia coli str. K-12 substr. MG1655

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3251

EcoGene

EcoGene:EG10608

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000601

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010661

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Ishihama, Y et al. (2008) Protein abundance profiling of the Escherichia coli cytosol. BMC Genomics 9 102 PubMed
  2. ↑ 2.0 2.1 Kruse, T et al. (2003) Dysfunctional MreB inhibits chromosome segregation in Escherichia coli. EMBO J. 22 5283-92 PubMed
  3. ↑ 3.0 3.1 3.2 Taniguchi, Y et al. (2010) Quantifying E. coli proteome and transcriptome with single-molecule sensitivity in single cells. Science 329 533-8 PubMed
  4. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  5. ↑ Wachi, M et al. (2006) Transcriptional analysis of the Escherichia coli mreBCD genes responsible for morphogenesis and chromosome segregation. Biosci. Biotechnol. Biochem. 70 2712-9 PubMed

Categories

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