Ishihama, Y, Schmidt, T, Rappsilber, J, Mann, M, Hartl, FU, Kerner, MJ and Frishman, D (2008) Protein abundance profiling of the Escherichia coli cytosol. BMC Genomics 9:102
Knowledge about the abundance of molecular components is an important prerequisite for building quantitative predictive models of cellular behavior. Proteins are central components of these models, since they carry out most of the fundamental processes in the cell. Thus far, protein concentrations have been difficult to measure on a large scale, but proteomic technologies have now advanced to a stage where this information becomes readily accessible.
Chromatography, Liquid/methods; Cytosol/metabolism; Escherichia coli/metabolism; Escherichia coli Proteins/analysis; Mass Spectrometry/methods; Proteomics/methods
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