entD:Gene: Difference between revisions

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entD over-expression
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Over-expression of entD can be used to substitute pfaE that is used to encode an Sfp-type posphopantetheinyl transferase.
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PMID:20636978
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Plasmid that over-expresses entD is [[pCA24N]]::entD


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Revision as of 20:46, 12 October 2012

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

entD

Mnemonic

Enterochelin

Synonyms

ECK0575, b0583, JW5085[1], JW5085


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

13.12 minutes 

MG1655: 609311..608682
<gbrowseImage> name=NC_000913:608682..609311 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 592795..592166
<gbrowseImage> name=NC_012967:592166..592795 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 511442..512071
<gbrowseImage> name=NC_012759:511442..512071 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 609311..608682
<gbrowseImage> name=AC_000091:608682..609311 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 548643..548014
<gbrowseImage> name=NC_010473:548014..548643 source=DH10B preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 661903..661274
<gbrowseImage> name=NC_010473:661274..661903 source=DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

entD-

Auxotrophies

Mutation prevents synthesis of enterochelin, Figure 3. Consequently rate of iron uptake is less than 10% of parent strain, Table 3.

PMID:4919746[2]

Mutation was induced by MNNG treatment.

entD-

Change in Protein Concentration

Mutation causes decreased levels of cytochrome b1 - Table 2.

PMID:4919746[2]



Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5085

Plasmid clone

Shigen

PMID:16769691[3]

Status:Clone OK

Primer 1:GCCGTCGATATGAAAACTACGCA

Primer 2:CCATCGTGTTGGCACAGCGTTAT

21A9S

Kohara Phage

Genobase

PMID:3038334[4]

2D12

Kohara Phage

Genobase

PMID:3038334[4]

purK79::Tn10

Linked marker

CAG12171 = CGSC7342[5]

est. P1 cotransduction: 6% [6]
Synonyms:purE79::Tn10

dsbG601::Tn10

Linked marker

CAG12149 = CGSC7346[5]

est. P1 cotransduction: 36% [6]
Synonyms:zbd-601::Tn10, zbe-601::Tn10


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

ECOCYC:EG10262

Escherichia coli str. K-12 substr. MG1655

EcoGene (EcoliWiki Page)

EcoGene:EG10262

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945194

Escherichia coli str. K-12 substr. MG1655


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Cox, GB et al. (1970) Mutations affecting iron transport in Escherichia coli. J. Bacteriol. 104 219-26 PubMed
  3. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  4. ↑ 4.0 4.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  5. ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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