ykfI:Gene: Difference between revisions

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Primer 2:CCTCGTACTACGTTGTTACGGCT  
Primer 2:CCTCGTACTACGTTGTTACGGCT  
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[[:Category:Kohara_Phage:8G4|8G4]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=128 Genobase]
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PMID:3038334
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est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/> <br />  
est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/> <br />  
Synonyms:lacI42::Tn10 nnnCAG18439 also carries lacZ118(0c) (CGSC).  
Synonyms:lacI42::Tn10 nnnCAG18439 also carries lacZ118(0c) (CGSC).  
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[[:Category:Kohara_Phage:8G4|8G4]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=128 Genobase]
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PMID:3038334


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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=G6120 EcoCyc:G6120]
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[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
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[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB4079 EchoBASE:EB4079]
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[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
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[http://chase.ou.edu/macro/searchlink.php?bnum=b0245 EcoliGenExpDB:b0245]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
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[http://ecogene.org/geneinfo.php?eg_id=EG14333 EcoGene:EG14333]
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[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
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[http://BioCyc.org/ECOO157/new-image?object=Z3165 ECOO157CYC:Z3165]
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[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0000836&formSubmitReturn=1 ASAP:ABE-0000836]
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Revision as of 22:31, 21 September 2011

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ykfI

Mnemonic

Systematic nomenclature

Synonyms

ECK0246, b0245, JW0234[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

5.66 minutes 

MG1655: 262893..262552
<gbrowseImage> name=NC_000913:262552..262893 source=MG1655 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 262551..262892
<gbrowseImage> name=NC_012759:262551..262892 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 262893..262552
<gbrowseImage> name=AC_000091:262552..262893 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 236997..236656
<gbrowseImage> name=NC_010473:236656..236997 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔykfI (Keio:JW0234)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC8469[3]

ΔykfI762::kan

PMID:16738554[2]

CGSC:105660


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0234

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCAAAACTTTACCTGCAATAAC

Primer 2:CCTCGTACTACGTTGTTACGGCT

8G4

Kohara Phage

Genobase

PMID:3038334[5]

proA81::Tn10

Linked marker

CAG18447 = CGSC7330[3]

est. P1 cotransduction: 91% [6]

lacI3042::Tn10

Linked marker

CAG18439 = CGSC7334[3]

est. P1 cotransduction: % [6]
Synonyms:lacI42::Tn10 nnnCAG18439 also carries lacZ118(0c) (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes
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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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