grpE:Gene: Difference between revisions

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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=438 Genobase]
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=438 Genobase]
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PMID:3038334
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[[:Category:Kohara_Phage:22D7|22D7]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=439 Genobase]
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PMID:3038334  
PMID:3038334  
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est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/> <br />  
est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/> <br />  
Synonyms:zfh-3131::Tn10, zfi-3131::Tn10  
Synonyms:zfh-3131::Tn10, zfi-3131::Tn10  
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[[:Category:Kohara_Phage:22D7|22D7]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=439 Genobase]
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PMID:3038334


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!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10416 EcoCyc:EG10416]
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[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
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[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0411 EchoBASE:EB0411]
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[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
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[http://chase.ou.edu/macro/searchlink.php?bnum=b2614 EcoliGenExpDB:b2614]
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[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
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[http://BioCyc.org/ECOL199310/new-image?object=C3135 ECOL199310CYC:C3135]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
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[http://ecogene.org/geneinfo.php?eg_id=EG10416 EcoGene:EG10416]
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[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
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[http://BioCyc.org/ECOO157/new-image?object=GRPE ECOO157CYC:GRPE]
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[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
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[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/660 CGSC:660]
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[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0008598&formSubmitReturn=1 ASAP:ABE-0008598]
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Revision as of 20:18, 21 September 2011

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

grpE

Mnemonic

Growth after phage induction

Synonyms

ECK2610, b2614, JW2594[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

59.23 minutes 

MG1655: 2748730..2748137
<gbrowseImage> name=NC_000913:2748137..2748730 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2672003..2671410
<gbrowseImage> name=NC_012967:2671410..2672003 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2633949..2634542
<gbrowseImage> name=NC_012759:2633949..2634542 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2749364..2748771
<gbrowseImage> name=AC_000091:2748771..2749364 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2840495..2839902
<gbrowseImage> name=NC_010473:2839902..2840495 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

grpEV108A

V108A

No effect in ability to interact with dnaK

seeded from UniProt:P09372

grpEG122D

G122D

Temperature-sensitive phenotype

seeded from UniProt:P09372

grpEL149A

L149A

No effect in ability to interact with dnaK

seeded from UniProt:P09372

grpEQ155A

Q155A

No effect in ability to interact with dnaK

seeded from UniProt:P09372

grpEI157A

I157A

No effect in ability to interact with dnaK

seeded from UniProt:P09372

grpEM159A

M159A

No effect in ability to interact with dnaK

seeded from UniProt:P09372

grpEM174A

M174A

No effect in ability to interact with dnaK

seeded from UniProt:P09372

grpER183A

R183A

Loss of ability to interact with dnaK

seeded from UniProt:P09372

grpER186A

R186A

No effect in ability to interact with dnaK

seeded from UniProt:P09372

grpEM189A

M189A

No effect in ability to interact with dnaK

seeded from UniProt:P09372

grpEV192A

V192A

Loss of ability to interact with dnaK

seeded from UniProt:P09372

grpER74A

R74A

Great decrease in ability to interact with dnaK

seeded from UniProt:P09372

grpEP151A

P151A

No effect in ability to interact with dnaK

seeded from UniProt:P09372

grpER73A

R73A

Great decrease in ability to interact with dnaK

seeded from UniProt:P09372

grpEK82A

K82A

Great decrease in ability to interact with dnaK

seeded from UniProt:P09372

grpEF86A

F86A

No effect in ability to interact with dnaK

seeded from UniProt:P09372

grpER104A

R104A

No effect in ability to interact with dnaK

seeded from UniProt:P09372

grpEE107A

E107A

No effect in ability to interact with dnaK

seeded from UniProt:P09372

grpE280

PMID:2424889[2]

CGSC:90840


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2594

Plasmid clone

Shigen

PMID:16769691[3]

Status:Clone OK

Primer 1:GCCAGTAGTAAAGAACAGAAAAC

Primer 2:CCAGCTTTTGCTTTCGCTACAG

E8F2

Kohara Phage

Genobase

PMID:3038334[4]

22D7

Kohara Phage

Genobase

PMID:3038334[4]

pheA18::Tn10

Linked marker

CAG12158 = CGSC7421[5]

est. P1 cotransduction: 69% [6]

srlD3131::Tn10

Linked marker

CAG18642 = CGSC7423[5]

est. P1 cotransduction: % [6]
Synonyms:zfh-3131::Tn10, zfi-3131::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes
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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Ang, D et al. (1986) Escherichia coli grpE gene codes for heat shock protein B25.3, essential for both lambda DNA replication at all temperatures and host growth at high temperature. J. Bacteriol. 167 25-9 PubMed
  3. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  4. ↑ 4.0 4.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  5. ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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