asnS:Gene: Difference between revisions

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Primer 2:CCGAAGCTGGCGTTACGCGGAGT  
Primer 2:CCGAAGCTGGCGTTACGCGGAGT  
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[[:Category:Kohara_Phage:E1D1|E1D1]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=218 Genobase]
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PMID:3038334
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[[:Category:Kohara_Phage:E1D1|E1D1]]
[[:Category:Kohara_Phage:11F5|11F5]]
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Kohara Phage
Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=218 Genobase]
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=219 Genobase]
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PMID:3038334  
PMID:3038334  

Revision as of 20:27, 2 September 2011

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

asnS

Mnemonic

Asparagine

Synonyms

ECK0921, b0930, JW0913, lcs, tss[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

21.27 minutes 

MG1655: 988208..986808
<gbrowseImage> name=NC_000913:986808..988208 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1006076..1004676
<gbrowseImage> name=NC_012967:1004676..1006076 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 889776..891176
<gbrowseImage> name=NC_012759:889776..891176 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 989407..988007
<gbrowseImage> name=AC_000091:988007..989407 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1042136..1040736
<gbrowseImage> name=NC_010473:1040736..1042136 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

986811

Edman degradation

PMID:2693216[2]
PMID:9298646[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

asnSP231L

P231L

Auxotrophies

(in temperature-sensitive mutant HO202)

Strain variation; seeded from UniProt:P0A8M0

asnSY426F

Y426F

Auxotrophies

No effect

seeded from UniProt:P0A8M0

asnSY426S

Y426S

Auxotrophies

15-fold increase in Km for ATP

seeded from UniProt:P0A8M0

asnS40(ts)

Auxotrophies

temperature sensitive

CGSC:7220


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0913

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCAGCGTTGTGCCTGTAGCCGA

Primer 2:CCGAAGCTGGCGTTACGCGGAGT

E1D1

Kohara Phage

Genobase

PMID:3038334[5]

zca-1230::Tn10

Linked marker

CAG18478 = CGSC7356[6]

est. P1 cotransduction: 18% [7]
Synonyms:zbj-1230::Tn10

zcb-3059::Tn10

Linked marker

CAG12094 = CGSC7358[6]

est. P1 cotransduction: 58% [7]

11F5

Kohara Phage

Genobase

PMID:3038334[5]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10094

EchoBASE (EcoliWiki Page)

EchoBASE:EB0092

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0930

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C1072

EcoGene (EcoliWiki Page)

EcoGene:EG10094

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:ASNS

CGSC (EcoliWiki Page)

CGSC:993

ASAP (EcoliWikiPage)

ASAP:ABE-0003162


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Anselme, J & Härtlein, M (1989) Asparaginyl-tRNA synthetase from Escherichia coli has significant sequence homologies with yeast aspartyl-tRNA synthetase. Gene 84 481-5 PubMed
  3. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. ↑ 5.0 5.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. ↑ 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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