PMID:2693216
| Citation |
Anselme, J and Härtlein, M (1989) Asparaginyl-tRNA synthetase from Escherichia coli has significant sequence homologies with yeast aspartyl-tRNA synthetase. Gene 84:481-5 |
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| Abstract |
The Escherichia coli asnS gene codes for asparaginyl-tRNA synthetase (NRSEC). We have sequenced the asnS region, including 382 bp of the 5'-untranslated region, 1398 bp of the coding region and 280 bp of the 3'-untranslated region. The DNA-derived NRSEC amino acid (aa) sequence was confirmed by direct aa sequencing of the N-terminal parts of the native protein and of a 28-kDa internal fragment generated by trypsin digestion. The asnS gene product has been purified to homogeneity using three chromatographic steps. Sequence comparison of the deduced NRSEC sequence with all aminoacyl-tRNA synthetase sequences showed significant homologies with the yeast aspartyl-tRNA synthetase and weaker relationships with other aminoacyl-tRNA synthetases for aa with an XAX codon. |
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| Keywords |
Amino Acid Sequence; Amino Acids/analysis; Amino Acyl-tRNA Synthetases/genetics; Aspartate-tRNA Ligase/genetics; Bacillus/enzymology; Bacillus/genetics; Base Sequence; Codon; DNA, Bacterial; DNA, Fungal; Escherichia coli/enzymology; Escherichia coli/genetics; Molecular Sequence Data; RNA, Transfer, Amino Acyl; Sequence Homology, Nucleic Acid; Yeasts/enzymology; Yeasts/genetics |
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