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Adding section & Genetic interactions table to Gene pages.
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|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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===Notes===
===Notes===


==Genetic Interactions==
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===Notes===


==Genetic Resources==
==Genetic Resources==

Revision as of 17:10, 24 June 2011

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

tsf

Mnemonic

Translation stable factor

Synonyms

ECK0169, b0170, JW0165[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

4.11 minutes 

MG1655: 190857..191708
<gbrowseImage> name=NC_000913:190857..191708 source=ecoli preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 193699..194550
<gbrowseImage> name=NC_012967:193699..194550 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 190856..191707
<gbrowseImage> name=NC_012759:190856..191707 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 190857..191708
<gbrowseImage> name=AC_000091:190857..191708 source=ecoli_W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 164961..165812
<gbrowseImage> name=NC_010473:164961..165812 source=ecoli_DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

190860

Edman degradation

PMID:8740179[2]
PMID:9298646[3]
PMID:9600841[4]
PMID:9740056[5]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

tsfF82A

F82A

2 to 3-fold less active in promoting GDP exchange and 6-fold less active in binding to EF-Tu

seeded from UniProt:P0A6P1

tsfH148A

H148A

At least 100-fold decrease in affinity between EF-Ts and EF-Tu and only small amount of GDP exchange activity

seeded from UniProt:P0A6P1

tsfK24A

K24A

No change in binding to EF-Tu and in promoting GDP exchange

seeded from UniProt:P0A6P1

tsfD81A

D81A

2 to 3-fold less active in promoting GDP exchange and slightly lower binding constant for interaction with EF- Tu

seeded from UniProt:P0A6P1

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0165

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCGCTGAAATTACCGCATCCCT

Primer 2:CCAGACTGCTTGGACATCGCAGC

zad-220::Tn10

Linked marker

CAG12025 = CGSC7326[7]

est. P1 cotransduction: 16% [8]

yafC502::Tn10

Linked marker

CAG18436 = CGSC7328[7]

est. P1 cotransduction: 17% [8]
Synonyms:zae-502::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11033

EchoBASE (EcoliWiki Page)

EchoBASE:EB1026

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0170

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0206

EcoGene (EcoliWiki Page)

EcoGene:EG11033

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:TSF

CGSC (EcoliWiki Page)

CGSC:64

ASAP (EcoliWikiPage)

ASAP:ABE-0000579


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Pasquali, C et al. (1996) Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database. Electrophoresis 17 547-55 PubMed
  3. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  4. ↑ Wilkins, MR et al. (1998) Protein identification with N and C-terminal sequence tags in proteome projects. J. Mol. Biol. 278 599-608 PubMed
  5. ↑ Tonella, L et al. (1998) '98 Escherichia coli SWISS-2DPAGE database update. Electrophoresis 19 1960-71 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 CGSC: The Coli Genetics Stock Center
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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