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|{{Sub}}[[#Sequence Features|Sequence Features]]
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|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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===Notes===
===Notes===


==Genetic Interactions==
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===Notes===


==Genetic Resources==
==Genetic Resources==

Revision as of 17:08, 24 June 2011

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

thrA

Mnemonic

Threonine

Synonyms

ECK0002, b0002, JW0001, Hs, thrA1, thrA2, thrD[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

0.01 minutes 

MG1655: 337..2799
<gbrowseImage> name=NC_000913:337..2799 source=ecoli preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 336..2798
<gbrowseImage> name=NC_012967:336..2798 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 337..2799
<gbrowseImage> name=NC_012759:337..2799 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 337..2799
<gbrowseImage> name=AC_000091:337..2799 source=ecoli_W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 337..2799
<gbrowseImage> name=NC_010473:337..2799 source=ecoli_DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

337

Edman degradation

PMID:354697[2]
PMID:4562989[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔthrA (Keio:JW0001)

deletion

deletion

PMID:16738554[4]

Shigen

thrA34::Tn10

Insertion at 425 bp in MG1655 (NC_000913)

adapted from Nichols et al.

thrA34::Tn10

CGSC:4834

thrA1101

CGSC:5343

thrA1015

CGSC:5352

thrA1100

CGSC:5879

thrA3092::Tn10kan

PMID:2540407[5] PMID:9829956[6]

CGSC:38986

thrA1016

CGSC:73731


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0001

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCCGAGTGTTGAAGTTCGGCGG

Primer 2:CCGACTCCTAACTTCCATGAGAG

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[8]

est. P1 cotransduction: 28% [9]
Synonyms:car-96::Tn10

mdoB202::Tn10

Linked marker

CAG18430 = CGSC7488[8]

est. P1 cotransduction: 12% [9]
Synonyms:zjj-202::Tn10, zji-202::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10998

EchoBASE (EcoliWiki Page)

EchoBASE:EB0991

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0002

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0003

EcoGene (EcoliWiki Page)

EcoGene:EG10998

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:THRA

CGSC (EcoliWiki Page)

CGSC:111

ASAP (EcoliWikiPage)

ASAP:ABE-0000008


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Cossart-Gheerbrant, P et al. (1978) The primary structure of Escherichia coli K12 aspartokinase I-homoserine dehydrogenase I-Isolation and characterisation of the peptides produced by cyanogen bromide. Biochim. Biophys. Acta 535 206-15 PubMed
  3. ↑ Falcoz-Kelly, F et al. (1972) Revised structure of aspartokinase I-homoserine dehydrogenase I of Escherichia coli K12. Evidence for four identical subunits. Eur. J. Biochem. 28 507-19 PubMed
  4. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. ↑ Singer, M et al. (1989) A collection of strains containing genetically linked alternating antibiotic resistance elements for genetic mapping of Escherichia coli. Microbiol. Rev. 53 1-24 PubMed
  6. ↑ Nichols, BP et al. (1998) Sequence analysis of Tn10 insertion sites in a collection of Escherichia coli strains used for genetic mapping and strain construction. J. Bacteriol. 180 6408-11 PubMed
  7. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. ↑ 8.0 8.1 CGSC: The Coli Genetics Stock Center
  9. ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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