acrA:Gene: Difference between revisions

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|{{Sub}}[[#Sequence Features|Sequence Features]]
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|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
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|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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===Notes===
===Notes===
AcrA mutants grow to higher cell density than wt <ref name='PMID: 16467145'/>
AcrA mutants grow to higher cell density than wt <ref name='PMID: 16467145'/>
==Genetic Interactions==
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===Notes===


==Genetic Resources==
==Genetic Resources==

Revision as of 16:10, 24 June 2011

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

acrA

Mnemonic

Acridine

Synonyms

ECK0457, b0463, JW0452, Mb, lir, mbl, mtcA, sipB, nbsA[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

10.42 minutes 

MG1655: 484843..483650
<gbrowseImage> name=NC_000913:483650..484843 source=ecoli preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 457686..456493
<gbrowseImage> name=NC_012967:456493..457686 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 386409..387602
<gbrowseImage> name=NC_012759:386409..387602 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 484843..483650
<gbrowseImage> name=AC_000091:483650..484843 source=ecoli_W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 424174..422981
<gbrowseImage> name=NC_010473:422981..424174 source=ecoli_DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔacrA (Keio:JW0452)

deletion

deletion

PMID:16738554[2]

Shigen

acrA1

CGSC:6294

acrA13

CGSC:7201


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Notes

AcrA mutants grow to higher cell density than wt [3]


Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0452

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCAACAAAAACAGAGGGTTTAC

Primer 2:CCAGACTTGGACTGTTCAGGCTG

zaj-3054::Tn10

Linked marker

CAG12017 = CGSC7339[5]

est. P1 cotransduction: 40% [6]
Synonyms:zba-3054::Tn10

gsk-3055::Tn10

Linked marker

CAG12154 = CGSC7341[5]

est. P1 cotransduction: 54% [6]
Synonyms:zbb-30-55::TnlO, zba-3055::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11703

EchoBASE (EcoliWiki Page)

EchoBASE:EB1654

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0463

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0581

EcoGene (EcoliWiki Page)

EcoGene:EG11703

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:ACRA

CGSC (EcoliWiki Page)

CGSC:1045

ASAP (EcoliWikiPage)

ASAP:ABE-0001603


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ Yang, S et al. (2006) Quorum sensing and multidrug transporters in Escherichia coli. Proc. Natl. Acad. Sci. U.S.A. 103 2386-91 PubMed
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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