moaC:Gene: Difference between revisions

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Fixing Blattner deletion strains in alleles table.
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0002673 moaC]
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0002673 moaC::Tn5KAN-2] (FB20237)
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Insertion at nt 387 in Plus orientation
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PMID:12519969
PMID:15262929
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[[asapstrains:ASAP|ASAP]]
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20237]
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At position 387 in Plus orientation, does not contain plasmid  [[pKD46]].<br />ASAP strain name: FB20237
does not contain [[pKD46]]  
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Revision as of 21:53, 9 March 2011

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

moaC

Mnemonic

Molybdenate

Synonyms

ECK0772, b0783, JW0766, chlA3[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

17.63 minutes 

MG1655: 817793..818278
<gbrowseImage> name=NC_000913:817793..818278 source=ecoli preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 811196..811681
<gbrowseImage> name=NC_012967:811196..811681 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 720761..721246
<gbrowseImage> name=NC_012759:720761..721246 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 818992..819477
<gbrowseImage> name=AC_000091:818992..819477 source=ecoli_W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 871721..872206
<gbrowseImage> name=NC_010473:871721..872206 source=ecoli_DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

817796

Edman degradation

PMID:8361352[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔmoaC (Keio:JW0766)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC8823[4]

moaC::Tn5KAN-2 (FB20237)

Insertion at nt 387 in Plus orientation

PMID:15262929[5]

E. coli Genome Project:FB20237

does not contain pKD46

moaCG52A

G52A

Reduced activity

seeded from UniProt:P0A738

moaCK67A

K67A

Reduced activity

seeded from UniProt:P0A738

moaCC76A

C76A

Reduced activity

seeded from UniProt:P0A738

moaCH77A

H77A

Reduced activity

seeded from UniProt:P0A738

moaCK131A

K131A

Reduced activity

seeded from UniProt:P0A738

moaCE112A

E112A

Reduced activity

seeded from UniProt:P0A738

moaCT117P

T117P

Strongly reduced activity

seeded from UniProt:P0A738

moaCD128A

D128A

Loss of activity

seeded from UniProt:P0A738

moaCG110A

G110A

Reduced activity

seeded from UniProt:P0A738

moaCK51A

K51A

Reduced activity

seeded from UniProt:P0A738

ΔmoaC755::kan

PMID:16738554[3]

CGSC:101902


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0766

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCTCGCAACTGACCCATATCAA

Primer 2:CCATCATCCGCTTCCACCTTAAA

nadA57::Tn10

Linked marker

CAG12147 = CGSC7351[4]

est. P1 cotransduction: 20% [7]

zbh-29::Tn10

Linked marker

CAG18493 = CGSC7353[4]

est. P1 cotransduction: 89% [7]
Synonyms:zbi-29::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11666

EchoBASE (EcoliWiki Page)

EchoBASE:EB1617

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0783

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0865

EcoGene (EcoliWiki Page)

EcoGene:EG11666

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:MOAC

CGSC (EcoliWiki Page)

CGSC:31225

ASAP (EcoliWikiPage)

ASAP:ABE-0002673


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Rivers, SL et al. (1993) Molecular genetic analysis of the moa operon of Escherichia coli K-12 required for molybdenum cofactor biosynthesis. Mol. Microbiol. 8 1071-81 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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