asnA:Gene: Difference between revisions
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Fixing Blattner deletion strains in alleles table. |
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0012242 asnA::Tn5KAN] | [http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0012242 asnA::Tn5KAN-I-SceI] (FB21433) | ||
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Insertion at nt 517 in Plus orientation | |||
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PMID: | PMID:15262929 | ||
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[ | [http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB21433] | ||
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contains [[pKD46]] | |||
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Revision as of 21:22, 9 March 2011
{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>
| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
asnA |
|---|---|
| Mnemonic |
Asparagine |
| Synonyms |
ECK3738, b3744, JW3722[1] |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
84.6 minutes |
MG1655: 3925178..3926170 |
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NC_012967: 3887516..3888508 |
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NC_012759: 3813511..3814503 |
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W3110 |
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W3110: 3709526..3708534 |
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DH10B: 4022762..4023754 |
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| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
ΔasnA (Keio:JW3722) |
deletion |
deletion |
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asnA::Tn5KAN-I-SceI (FB21433) |
Insertion at nt 517 in Plus orientation |
contains pKD46 | |||||
|
asnA31 |
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ΔasnA773::kan |
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| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW3722 |
Plasmid clone |
Status:Clone OK Primer 1:GCCAAAACCGCTTACATTGCCAA Primer 2:CCaAGCAGAGAAGGGACGCTCTC | |
|
zid-501::Tn10 |
Linked marker |
est. P1 cotransduction: 9% [6] | |
|
Linked marker |
est. P1 cotransduction: 86% [6] | ||
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
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| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
- ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).