murE:Gene: Difference between revisions

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Revision as of 18:18, 17 June 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

murE

Mnemonic

Murein

Synonyms

ECK0086, b0085, JW0083[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

2.01 minutes 

MG1655: 93166..94653
gbrowse_img.php?coord=93166..94653&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 95970..97457
gbrowse_img.php?coord=95970..97457&genome=REL606&name=NC_012967&trackset=0&width=300&.png

BW2952

NC_012759: 93165..94652
gbrowse_img.php?coord=93165..94652&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

W3110

 

W3110: 93166..94653
gbrowse_img.php?coord=93166..94653&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 67270..68757
gbrowse_img.php?coord=67270..68757&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

93169

Edman degradation

PMID:2198024[2]
PMID:2269304[3]



Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

murEA495S

A495S

(in murE1)

Strain variation; seeded from UniProt:P22188

murEE344K

E344K

(in murE1)

Strain variation; seeded from UniProt:P22188

murE1

CGSC:9851



Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0083

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCGCAGATCGTAATTTGCGCGA

Primer 2:CCTGCAATCACCCCCAGCAGACG

leuO3051::Tn10

Linked marker

CAG12095 = CGSC7324[5]

est. P1 cotransduction: 72% [6]
Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).

zad-220::Tn10

Linked marker

CAG12025 = CGSC7326[5]

est. P1 cotransduction: 7% [6]


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10621

EchoBASE (EcoliWiki Page)

EchoBASE:EB0616

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0085

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0103

EcoGene (EcoliWiki Page)

EcoGene:EG10621

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:MURE

CGSC (EcoliWiki Page)

CGSC:475

ASAP (EcoliWikiPage)

ASAP:ABE-0000311



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Michaud, C et al. (1990) Revised interpretation of the sequence containing the murE gene encoding the UDP-N-acetylmuramyl-tripeptide synthetase of Escherichia coli. Biochem. J. 269 277-8 PubMed
  3. ↑ Michaud, C et al. (1990) Over-production, purification and properties of the uridine-diphosphate-N-acetylmuramoyl-L-alanyl-D-glutamate: meso-2,6-diaminopimelate ligase from Escherichia coli. Eur. J. Biochem. 194 853-61 PubMed
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. ↑ 5.0 5.1 CGSC: The Coli Genetics Stock Center
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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