arsB:Gene Product(s): Difference between revisions

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!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
!|Qualifier!!GO ID!!GO term name!!Reference(s)!!Evidence Code!!with/from!!Aspect!!Notes!!Status
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GO:0016020
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membrane
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GO_REF:0000004<br />GO_REF:0000004
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IEA: Inferred from Electronic Annotation
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SP_KW:KW-0397<br />SP_KW:KW-0472
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C
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Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
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complete
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GO:0016021
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integral to membrane
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GO_REF:0000002<br />GO_REF:0000004
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IEA: Inferred from Electronic Annotation
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InterPro:IPR000802<br />SP_KW:KW-0812
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C
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Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
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complete
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GO:0008490
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arsenite secondary active transmembrane transporter activity
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PMID:14970228
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IDA: Inferred from Direct Assay
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F
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Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
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complete
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GO:0015699
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antimonite transport
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PMID:14970228
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IDA: Inferred from Direct Assay
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P
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Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
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complete
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GO:0015700
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arsenite transport
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PMID:14970228
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IDA: Inferred from Direct Assay
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P
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Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
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complete
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GO:0042960
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antimonite secondary active transmembrane transporter activity
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PMID:14970228
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IDA: Inferred from Direct Assay
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F
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Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
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complete
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GO:0046685
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response to arsenic
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PMID:7860609
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IEP: Inferred from Expression Pattern
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P
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Seeded from EcoCyc 12.5 <ref name = 'LIB:EcoCyc12.5'/>
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complete
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GO:0005886
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plasma membrane
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GO_REF:0000004<br />GO_REF:0000004<br />GO_REF:0000023
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IEA: Inferred from Electronic Annotation
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SP_KW:KW-0997<br />SP_KW:KW-1003<br />SP_SL:SL-0037
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C
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Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
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complete
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GO:0006810
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transport
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GO_REF:0000004
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IEA: Inferred from Electronic Annotation
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SP_KW:KW-0813
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P
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Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
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complete
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GO:0015105
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arsenite transmembrane transporter activity
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GO_REF:0000002
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IEA: Inferred from Electronic Annotation
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InterPro:IPR000802
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F
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Seeded from EcoCyc <ref name = 'LIB:EcoCyc13.0'/>
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complete
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GO:0006805
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xenobiotic metabolic process
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P
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Seeded from EcoCyc 11.1<ref name='LIB:EcoCyc11.1'/>.
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required fields missing 
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Revision as of 20:42, 10 June 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

ArsB

Synonyms

arsenite/antimonite transporter[1], B3502[2][1], ArsF[2][1], F172[2][1]

Product description

ArsB[2][3];

Component of arsenate Ars transporter[2][3]

Arsenite pump, inner membrane; resistance to arsenate, arsenite, and antimonite[4]

EC number (for enzymes)


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Notes

Function

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Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference(s) Evidence Code with/from Aspect Notes Status
GO:0005887

PMID:20196076[5]

IDA: Inferred from Direct Assay

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of arsenate Ars transporter

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Source Notes

plasma membrane

C-terminus localized to the periplasm with 11 predicted transmembrane domains

Daley et al. (2005) [6]

plasma membrane

From EcoCyc[3]


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Notes

Structure and Physical Properties

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Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Sequence

at EcoCyc 

MFYPGGIMLL AGAIFVLTIV LVIWQPKGLG IGWSATLGAV LALVTGVVHP GDIPVVWNIV
WNATAAFIAV IIISLLLDES GFFEWAALHV SRWGNGRGRL LFTWIVLLGA AVAALFANDG
AALILTPIVI AMLLALGFSK GTTLAFVMAA GFIADTASLP LIVSNLVNIV SADFFGLGFR
EYASVMVPVD IAAIVATLVM LHLYFRKDIP QNYDMALLKS PAEAIKDPAT FKTGWVVLLL
LLVGFFVLEP LGIPVSAIAA VGALILFVVA KRGHAINTGK VLRGAPWQIV IFSLGMYLVV
YGLRNAGLTE YLSGVLNVLA DNGLWAATLG TGFLTAFLSS IMNNMPTVLV GALSIDGSTA
SGVIKEAMVY ANVIGCDLGP KITPIGSLAT LLWLHVLSQK NMTISWGYYF RTGIIMTLPV
LFVTLAALAL RLSFTL
Length

436

Mol. Wt

46.264 kDa

pI

8.8 (calculated)

Extinction coefficient

80,900 - 81,025 (calc based on 10 Y, 12 W, and 1 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References
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<motif_map/>

tmhmm.php?gene=arsB&show=pic&.jpg
Click the image to view raw output from TMHMM.

Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=arsB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:ARSF-MONOMER

UniProt (EcoliWiki Page)

UniProt:P0AB93

PFAM (EcoliWiki Page)

PFAM:PF02040

RefSeq (EcoliWiki Page)

RefSeq:NP_417959


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 2.4 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Hsieh, JM et al. (2010) Bridging the gap: a GFP-based strategy for overexpression and purification of membrane proteins with intra and extracellular C-termini. Protein Sci. 19 868-80 PubMed
  6. ↑ Daley, DO et al. (2005) Global topology analysis of the Escherichia coli inner membrane proteome. Science 308 1321-3 PubMed

Categories

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