ptrA:Gene: Difference between revisions

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!|Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes


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''&#916;ptrA746::kan''
&Delta;ptr::kan
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deletion
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[[Biolog]]:respiration
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unable to respire [[Carbon sources|L-Aspartate]]
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PMID:16738554
PMID:16095938
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[[cgscmutation:113619|CGSC:113619]]
 
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&Delta;ptr::kan
''&#916;ptrA746::kan''
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deletion
 
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[[Biolog]]:respiration
 
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unable to respire [[Carbon sources|L-Aspartate]]
 
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PMID:16095938
PMID:16738554
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[[cgscmutation:113619|CGSC:113619]]
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Revision as of 23:19, 10 May 2010

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ptrA

Mnemonic

Protease III

Synonyms

ptr, ECK2817, b2821, JW2789[1][2]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

63.67 minutes 

MG1655: 2956906..2954018
gbrowse_img.php?coord=2954018..2956906&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 2850615..2853503
gbrowse_img.php?coord=2850615..2853503&genome=REL606&name=NC_012967&trackset=0&width=300&.png

BW2952

NC_012759: 2841166..2844054
gbrowse_img.php?coord=2841166..2844054&genome=BW2952&name=NC_012759&trackset=0&width=300&.png

W3110

 

W3110: 2957540..2954652
gbrowse_img.php?coord=2954652..2957540&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 3050776..3047888
gbrowse_img.php?coord=3047888..3050776&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ptrAH88R

H88R

Loss of activity and of Zn-binding

seeded from UniProt:P05458

ptrAE162Q

E162Q

20% loss of activity

seeded from UniProt:P05458

ptrAE169Q

E169Q

Loss of activity and of Zn-binding

seeded from UniProt:P05458

ptrAE204Q

E204Q

No loss of activity

seeded from UniProt:P05458

ptrAE91Q

E91Q

Loss of activity

seeded from UniProt:P05458

ptrAH92R

H92R

Loss of activity and of Zn-binding

seeded from UniProt:P05458

Δptr::kan

deletion

Biolog:respiration

unable to respire Acetate

PMID:16095938[3]

Δptr::kan

deletion

Biolog:respiration

unable to respire Bromosuccinate

PMID:16095938[3]

Δptr::kan

deletion

Biolog:respiration

unable to respire L-Asparagine

PMID:16095938[3]

Δptr::kan

deletion

Biolog:respiration

unable to respire L-Aspartate

PMID:16095938[3]

ΔptrA746::kan

PMID:16738554[4]

CGSC:113619



Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691[5]

Status:

Primer 1:

Primer 2:


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10786

EchoBASE (EcoliWiki Page)

EchoBASE:EB0779

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2821

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3415

EcoGene (EcoliWiki Page)

EcoGene:EG10786

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:PTR

CGSC (EcoliWiki Page)

CGSC:351

ASAP (EcoliWikiPage)

ASAP:ABE-0009252



Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 3.2 3.3 Ito, M et al. (2005) Functional analysis of 1440 Escherichia coli genes using the combination of knock-out library and phenotype microarrays. Metab. Eng. 7 318-27 PubMed
  4. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed

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