dnaK:Gene: Difference between revisions

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!align=left {{table_heading_style}} |Standard name
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''dnaK''
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!align=left {{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Mnemonic
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DNA
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!align=left {{table_heading_style}} |Synonyms
!align=left {{table_heading_style}} |Synonyms
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''ECK0014, b0014, JW0013, grpF, seg, lop, groPAB, groPC, groPF, grpC, dnaL, groP, groPA''<ref name='LIB:Riley_2006'/><ref name='LIB:EcoCyc10.6'/>


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!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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''dnaK756''(ts)
dnaKG468D
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G468D
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temperature sensitive
In mutant dnaK756(TS); confers temperature sensitivity
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[[cgscmutation:6707|CGSC:6707]]
 
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seeded from [http://www.uniprot.org/uniprot/P0A6Y8 UniProt:P0A6Y8]
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''&#916;dnaK734::kan''
''dnaK756''(ts)
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temperature sensitive
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PMID:16738554
[[cgscmutation:6707|CGSC:6707]]
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[[cgscmutation:100628|CGSC:100628]]
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dnaKG468D
''&#916;dnaK734::kan''
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G468D
 
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In mutant dnaK756(TS); confers temperature sensitivity
 
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PMID:16738554
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[[cgscmutation:100628|CGSC:100628]]
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seeded from [http://www.uniprot.org/uniprot/P0A6Y8 UniProt:P0A6Y8]


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Revision as of 21:06, 25 February 2010

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dnaK

Mnemonic

DNA

Synonyms

ECK0014, b0014, JW0013, grpF, seg, lop, groPAB, groPC, groPF, grpC, dnaL, groP, groPA[1][2]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

0.26 minutes 

MG1655: 12163..14079
gbrowse_img.php?coord=12163..14079&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 12161..14077
gbrowse_img.php?coord=12161..14077&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 12163..14079
gbrowse_img.php?coord=12163..14079&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 12163..14079
gbrowse_img.php?coord=12163..14079&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔdnaK (Keio:JW0013)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC8342[4]

dnaKG32D

G32D

In SEG-1 and dnaK756(TS); confers temperature sensitivity

seeded from UniProt:P0A6Y8

dnaKV436I

V436I

In mutant SEG-2; confers temperature sensitivity

seeded from UniProt:P0A6Y8

dnaKG455D

G455D

In mutant dnaK756(TS); confers temperature sensitivity

seeded from UniProt:P0A6Y8

dnaKG468D

G468D

In mutant dnaK756(TS); confers temperature sensitivity

seeded from UniProt:P0A6Y8

dnaK756(ts)

temperature sensitive

CGSC:6707

ΔdnaK734::kan

PMID:16738554[3]

CGSC:100628


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0013

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCGGTAAAATAATTGGTATCGA

Primer 2:CCTTTTTTGTCTTTGACTTCTTC

thrA34::Tn10

Linked marker

CAG18442 = CGSC7320[4]

est. P1 cotransduction: 66% [6]
Synonyms:thr-34::Tn10

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[4]

est. P1 cotransduction: 48% [6]
Synonyms:car-96::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10241

EchoBASE (EcoliWiki Page)

EchoBASE:EB0237

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0014

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C0019

EcoGene (EcoliWiki Page)

EcoGene:EG10241

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:DNAK

CGSC (EcoliWiki Page)

CGSC:844

ASAP (EcoliWikiPage)

ASAP:ABE-0000052


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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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