ispG:Gene: Difference between revisions

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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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ispGG328D
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G328D
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Loss of activity
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seeded from [http://www.uniprot.org/uniprot/P62620 UniProt:P62620]
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ispGG328D
ispGR335C
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G328D
R335C
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Revision as of 17:43, 18 February 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ispG

Mnemonic
Synonyms

ECK2511, b2515, JW2499, gcpE[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

56.87 minutes 

MG1655: 2639826..2638708
gbrowse_img.php?coord=2638708..2639826&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 2562623..2561505
gbrowse_img.php?coord=2561505..2562623&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 2640460..2639342
gbrowse_img.php?coord=2639342..2640460&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 2731591..2730473
gbrowse_img.php?coord=2730473..2731591&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ispGG328D

G328D

Loss of activity

seeded from UniProt:P62620

ispGG322D

G322D

Loss of activity

seeded from UniProt:P62620

ispGR335C

R335C

Loss of activity

seeded from UniProt:P62620


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2499

Plasmid clone

Shigen

PMID:16769691[2]

Status:Clone OK

Primer 1:GCCCATAACCAGGCTCCAATTCA

Primer 2:CCTTTTTCAACCTGCTGAACGTC

purC80::Tn10

Linked marker

CAG18470 = CGSC7413[3]

est. P1 cotransduction: 14% [4]

zfh-208::Tn10

Linked marker

CAG18481 = CGSC7417[3]

est. P1 cotransduction: 40% [4]
Synonyms:zff-208::Tn10


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10370

EchoBASE (EcoliWiki Page)

EchoBASE:EB0365

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2515

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3037

EcoGene (EcoliWiki Page)

EcoGene:EG10370

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:GCPE

CGSC (EcoliWiki Page)

CGSC:32909

ASAP (EcoliWikiPage)

ASAP:ABE-0008281



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  3. ↑ 3.0 3.1 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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