ispG:Gene

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Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

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Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ispG

Mnemonic

Isoprenoid

Synonyms

ECK2511, b2515, JW2499, gcpE[1], gcpE

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Notes

Location(s) and DNA Sequence

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<protect> See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

56.87 minutes 

MG1655: 2639826..2638708
<gbrowseImage> name=NC_000913:2638708..2639826 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2562623..2561505
<gbrowseImage> name=NC_012967:2561505..2562623 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2524513..2525631
<gbrowseImage> name=NC_012759:2524513..2525631 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2640460..2639342
<gbrowseImage> name=NC_007779:2639342..2640460 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2731591..2730473
<gbrowseImage> name=NC_010473:2730473..2731591 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ispGG328D

G328D

Loss of activity

seeded from UniProt:P62620

ispGR335C

R335C

Loss of activity

seeded from UniProt:P62620

ispGG322D

G322D

Loss of activity

seeded from UniProt:P62620

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes

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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2499

Plasmid clone

Shigen

PMID:16769691[2]

Status:Clone OK

Primer 1:GCCCATAACCAGGCTCCAATTCA

Primer 2:CCTTTTTCAACCTGCTGAACGTC

8E3

Kohara Phage

Genobase

PMID:3038334[3]

2D5

Kohara Phage

Genobase

PMID:3038334[3]

7F8

Kohara Phage

Genobase

PMID:3038334[3]

purC80::Tn10

Linked marker

CAG18470 = CGSC7413[4]

est. P1 cotransduction: 14% [5]

zfh-208::Tn10

Linked marker

CAG18481 = CGSC7417[4]

est. P1 cotransduction: 40% [5]
Synonyms:zff-208::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10370

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10370

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000363

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:946991

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0365

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0008281

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  3. 3.0 3.1 3.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  4. 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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