aspA:Gene: Difference between revisions
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Loss of activity | Loss of activity | ||
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seeded from [http://www.uniprot.org/uniprot/P0AC38 UniProt:P0AC38] | |||
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aspAD10N | |||
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D10N | |||
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Reduces Kcal by 80%. Increases KM for aspartic acid 3-fold | |||
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aspAR15A | |||
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R15A | |||
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No effect on Kcat. Increases KM for aspartic acid 2.5-fold | |||
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Revision as of 17:26, 18 February 2010
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Alleles and Phenotypes | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
aspA |
|---|---|
| Mnemonic | |
| Synonyms |
ECK4133, b4139, JW4099[1] |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
94.08 minutes, 94.08 minutes |
MG1655: 4366350..4364914 |
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NC_012967: 4347048..4345612 |
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W3110 |
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W3110: 4373007..4371571 |
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DH10B: 4466712..4465276 |
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| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature type | Location | Description |
|---|---|---|
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
aspAD10A |
D10A |
Loss of activity |
seeded from UniProt:P0AC38 | ||||
|
aspAD10N |
D10N |
Reduces Kcal by 80%. Increases KM for aspartic acid 3-fold |
seeded from UniProt:P0AC38 | ||||
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ΔaspA (Keio:JW4099) |
deletion |
deletion |
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aspA22 |
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aspA23 |
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ΔaspA765::kan |
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|
aspAR15A |
R15A |
No effect on Kcat. Increases KM for aspartic acid 2.5-fold |
seeded from UniProt:P0AC38 | ||||
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW4099 |
Plasmid clone |
Status:Clone OK Primer 1:GCCTCAAACAACATTCGTATCGA Primer 2:CCtTGTTCGCTTTCATCAGTATA | |
|
Linked marker |
est. P1 cotransduction: 76% [5] | ||
|
Linked marker |
est. P1 cotransduction: 4% [5] | ||
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
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| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
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References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
- ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).