pitA:Gene: Difference between revisions

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!align=left |Standard name
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''pitA''  
''pitA''
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!align=left |Mnemonic
!align=left {{table_heading_style}} |Mnemonic
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Pi transport
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!align=left |Synonyms
!align=left {{table_heading_style}} |Synonyms
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''ECK3478, b3493, JW3460, pit, G442''<ref name='LIB:Riley_2006'/><ref name='LIB:EcoCyc10.6'/>
''ECK3478, b3493, JW3460, pit, G442''<ref name='LIB:Riley_2006'/><ref name='LIB:EcoCyc10.6'/>
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!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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Revision as of 21:34, 25 February 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

pitA

Mnemonic

Pi transport

Synonyms

ECK3478, b3493, JW3460, pit, G442[1][2]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

78.36 minutes 

MG1655: 3635665..3637164
gbrowse_img.php?coord=3635665..3637164&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 3568331..3569830
gbrowse_img.php?coord=3568331..3569830&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 4002773..4001274
gbrowse_img.php?coord=4001274..4002773&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 3733410..3734909
gbrowse_img.php?coord=3733410..3734909&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔpitA (Keio:JW3460)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC10563[4]

pitA10

PMID:4570598[5] PMID:4568763[6]

CGSC:5633

pitA1

CGSC:15787

ΔpitA749::kan

PMID:16738554[3]

CGSC:102374


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3460

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCCTACATTTGTTTGCTGGCCT

Primer 2:CCaAGGAACTGCAAGGAGAGCCA

zhg-50::Tn10

Linked marker

CAG18450 = CGSC7450[4]

est. P1 cotransduction: 14% [8]
Synonyms:zhf-50::Tn10, zhf-5::Tn10

zic-4901::Tn10

Linked marker

CAG18492 = CGSC7457[4]

est. P1 cotransduction: % [8]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG12230

EchoBASE (EcoliWiki Page)

EchoBASE:EB2142

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3493

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4291

EcoGene (EcoliWiki Page)

EcoGene:EG12230

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:PITA

ASAP (EcoliWikiPage)

ASAP:ABE-0011407


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ Willsky, GR et al. (1973) Inorganic phosphate transport in Escherichia coli: involvement of two genes which play a role in alkaline phosphatase regulation. J. Bacteriol. 113 529-39 PubMed
  6. ↑ Bachmann, BJ (1972) Pedigrees of some mutant strains of Escherichia coli K-12. Bacteriol Rev 36 525-57 PubMed
  7. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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