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!align=left {{table_heading_style}} |Standard name
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''ppk''  
''ppk''  
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!align=left {{table_heading_style}} |Mnemonic
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!align=left {{table_heading_style}} |Synonyms
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''ECK2497, b2501, JW2486''<ref name='LIB:Riley_2006'/>
''ECK2497, b2501, JW2486''<ref name='LIB:Riley_2006'/>


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!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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[[REL606]]
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NC_012967: 2543863..2545929<br />{{GBrowseFigureByCoord|2543863|2545929|REL606|NC_012967}}
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!|Feature type!!Location!!Description
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Synonyms:zff-208::Tn10  
Synonyms:zff-208::Tn10  


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Revision as of 05:20, 9 January 2010

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ppk

Mnemonic
Synonyms

ECK2497, b2501, JW2486[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

56.49 minutes, 56.49 minutes 

MG1655: 2621066..2623132
gbrowse_img.php?coord=2621066..2623132&genome=ecoli&name=MG1655&trackset=0&width=300&.png

REL606

NC_012967: 2543863..2545929
gbrowse_img.php?coord=2543863..2545929&genome=REL606&name=NC_012967&trackset=0&width=300&.png

W3110

 

W3110: 2621700..2623766
gbrowse_img.php?coord=2621700..2623766&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 2712831..2714897
gbrowse_img.php?coord=2712831..2714897&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δppk (Keio:JW2486)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9985[3]

ppk

PMID:12519969[4]

ASAP

At position 1370 in Minus orientation, contains plasmid pKD46.
ASAP strain name: FB20830

Δppk-749::kan

PMID:16738554[2]

CGSC:102436


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2486

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCGGTCAGGAAAAGCTATACAT

Primer 2:CCTTCAGGTTGTTCGAGTGATTT

purC80::Tn10

Linked marker

CAG18470 = CGSC7413[3]

est. P1 cotransduction: 35% [6]

zfh-208::Tn10

Linked marker

CAG18481 = CGSC7417[3]

est. P1 cotransduction: 16% [6]
Synonyms:zff-208::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11510

EchoBASE (EcoliWiki Page)

EchoBASE:EB1472

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2501

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3019

EcoGene (EcoliWiki Page)

EcoGene:EG11510

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:PPK

CGSC (EcoliWiki Page)

CGSC:32894

ASAP (EcoliWikiPage)

ASAP:ABE-0008235


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Glasner, JD et al. (2003) ASAP, a systematic annotation package for community analysis of genomes. Nucleic Acids Res. 31 147-51 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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