pta:Gene: Difference between revisions

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Revision as of 23:50, 12 December 2009

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

pta

Mnemonic
Synonyms

ECK2291, b2297, JW2294[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

52 minutes 

MG1655: 2412769..2414913
gbrowse_img.php?coord=2412769..2414913&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 2420193..2422337
gbrowse_img.php?coord=2420193..2422337&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

DH10B

DH10B: 2504534..2506678
gbrowse_img.php?coord=2504534..2506678&genome=ecoli_DH10B&name=DH10B&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δpta (Keio:JW2294)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9844[3]

pta

PMID:12519969[4]

ASAP

At position 1310 in Minus orientation, does not contain plasmid pKD46.
ASAP strain name: FB20752

pta

PMID:12519969[4]

ASAP

At position 1310 in Minus orientation, contains plasmid pKD46 Insertion site is approximate.
ASAP strain name: FB20753

pta-39

CGSC:7369

pta-200

PMID:6259116[5]

CGSC:25449

Δpta-779::kan

PMID:16738554[2]

CGSC:102512


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2294

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCTCCCGTATTATTATGCTGAT

Primer 2:CCtTGCTGCTGTGCAGACTGAAT

zfb-223::Tn10

Linked marker

CAG18484 = CGSC7406[3]

est. P1 cotransduction: 42% [7]
Synonyms:zej-223::Tn10

zfd-1::Tn10

Linked marker

CAG18467 = CGSC7408[3]

est. P1 cotransduction: % [7]
Synonyms:zfb-1::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG20173

EchoBASE (EcoliWiki Page)

EchoBASE:EB4147

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2297

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C2840

EcoGene (EcoliWiki Page)

EcoGene:EG20173

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:PTA

CGSC (EcoliWiki Page)

CGSC:353

ASAP (EcoliWikiPage)

ASAP:ABE-0007582


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Notes

Links

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Name URL Comments
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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ 4.0 4.1 Glasner, JD et al. (2003) ASAP, a systematic annotation package for community analysis of genomes. Nucleic Acids Res. 31 147-51 PubMed
  5. ↑ LeVine, SM et al. (1980) Isolation and Characterization of acetate kinase and phosphotransacetylase mutants of Escherichia coli and Salmonella typhimurium. J. Bacteriol. 143 1081-5 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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