amn:Gene: Difference between revisions

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PMID:16738554
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[[cgscmutation:99996|CGSC:99996]]
[[asapstrains:ASAP|ASAP]]
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At position 239 in Minus orientation, contains plasmid [[pKD46]].<br />ASAP strain name: FB20556
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PMID:12519969
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[[asapstrains:ASAP|ASAP]]
[[cgscmutation:99996|CGSC:99996]]
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At position 239 in Minus orientation, contains plasmid [[pKD46]].<br />ASAP strain name: FB20556
   


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[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:amn genes]]
[[Category:amn genes]][[Category:Gene_List:DH10B]]

Revision as of 15:49, 6 April 2009

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

amn

Mnemonic
Synonyms

ECK1977, b1982, JW1963[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

44.25 minutes 

MG1655: 2053085..2054539
gbrowse_img.php?coord=2052085..2055539&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 2057198..2058652
gbrowse_img.php?coord=2056198..2059652&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature type Location Description
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δamn (Keio:JW1963)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC9628[3]

amn

PMID:12519969[4]

ASAP

At position 239 in Minus orientation, contains plasmid pKD46.
ASAP strain name: FB20556

Δamn-756::kan

PMID:16738554[2]

CGSC:99996


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1963

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCAATAATAAGGGCTCCGGTCT

Primer 2:CCTCGGAACGGCGGCTCATTAAA

zed-3069::Tn10

Linked marker

CAG18451 = CGSC7395[3]

est. P1 cotransduction: 56% [6]

zef-3129::Tn10

Linked marker

CAG12099 = CGSC7397[3]

est. P1 cotransduction: 31% [6]
Synonyms:zee-3129::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10039

EchoBASE (EcoliWiki Page)

EchoBASE:EB0037

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1982

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C2444

EcoGene (EcoliWiki Page)

EcoGene:EG10039

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:AMN

CGSC (EcoliWiki Page)

CGSC:17764

ASAP (EcoliWikiPage)

ASAP:ABE-0006579


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Notes

Links

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Name URL Comments
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References

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  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Glasner, JD et al. (2003) ASAP, a systematic annotation package for community analysis of genomes. Nucleic Acids Res. 31 147-51 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).


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