gapC:Gene: Difference between revisions

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Primer 2:  
Primer 2:  
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[[ompN:Gene|zhj-3076::Tn10]]
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Linked marker
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CAG18640 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40323 CGSC7453]<ref name='LIB:cgsc'/>
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est. P1 cotransduction: 7% <ref name='LIB:Nichols_linked_Tn'/>
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[[trg:Gene|trg-2::Tn10]]
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Linked marker
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CAG12026 = [http://cgsc2.biology.yale.edu/Strain.php?ID=39819 CGSC7376]<ref name='LIB:cgsc'/>
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est. P1 cotransduction: 82% <ref name='LIB:Nichols_linked_Tn'/>
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Revision as of 22:00, 28 July 2007

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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Standard name

gapC_1

Mnemonic
Synonyms

gapC, ECK1409, b1416, JW1413, b1417, JW5906, b4493, JW5906+JW1413, gapC_2[1][2][3]

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Notes

Location(s) and DNA Sequence

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Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

32.07 minutes 

MG1655: 1487988..1487737
gbrowse_img.php?coord=1486737..1488988&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 1491678..1491427
gbrowse_img.php?coord=1490427..1492678&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

MG1655

32.07 minutes 

MG1655: 1488737..1487985
gbrowse_img.php?coord=1486985..1489737&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 1492427..1491675
gbrowse_img.php?coord=1490675..1493427&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

MG1655

 

MG1655: 1488737..1487737
gbrowse_img.php?coord=1486737..1489737&genome=ecoli&name=MG1655&trackset=0&width=300&.png

W3110

 

W3110: 1492427..1491427
gbrowse_img.php?coord=1490427..1493427&genome=ecoli_W3110&name=W3110&trackset=0&width=300&.png

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Notes

Sequence Features

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Feature type Location Description
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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Notes

Alleles and Phenotypes

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Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability

ΔgapC_1 (Keio:)

deletion

deletion

PMID:16738554[4]

Shigen

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Notes

Genetic Resources

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Resource Resource Type Source Notes/Reference

Plasmid clone

Shigen

PMID:16769691[5]

Status:

Primer 1:

Primer 2:

zhj-3076::Tn10

Linked marker

CAG18640 = CGSC7453[6]

est. P1 cotransduction: 7% [7]

trg-2::Tn10

Linked marker

CAG12026 = CGSC7376[6]

est. P1 cotransduction: 82% [7]

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Notes

Accessions in Other Databases

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Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:G8205

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b1417

EcoGene (EcoliWiki Page)

EcoGene:EG12103

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:GAPC

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Notes

Links

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Name URL Comments
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See Help:Links_table for how to enter or edit information in this section of EcoliWiki.

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).