fabI:Gene: Difference between revisions

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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11528 ECOCYC:EG11528]
EcoCyc:EG11528
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[EcoGene]]
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[http://ecogene.org/geneinfo.php?eg_id=EG11528 EcoGene:EG11528]
EcoGene:EG11528
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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Escherichia coli str. K-12 substr. MG1655  
Escherichia coli str. K-12 substr. MG1655  
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[[RegulonDB]]
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RegulonDB:ECK120001482
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[EchoBASE]]
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EchoBASE:EB1490
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[ASAP]]
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ASAP:ABE-0004327
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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Revision as of 07:12, 27 May 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

fabI

Mnemonic

Fatty acid biosynthesis

Synonyms

ECK1283, b1288, JW1281, envM, gts, qmeA, G482[1][2]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

29.06 minutes 

MG1655: 1349063..1348275
<gbrowseImage> name=NC_000913:1348275..1349063 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1349408..1348620
<gbrowseImage> name=NC_012967:1348620..1349408 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 1239124..1239912
<gbrowseImage> name=NC_012759:1239124..1239912 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1352753..1351965
<gbrowseImage> name=AC_000091:1351965..1352753 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1438459..1437671
<gbrowseImage> name=NC_010473:1437671..1438459 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1348278

Edman degradation

PMID:1364817[3]
PMID:8119879[4]
PMID:9298646[5]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

fabIG93S

G93S

Diazaborine resistance

seeded from UniProt:P0AEK4

fabIS241F

S241F

Produces temperature-sensitive phenotype

seeded from UniProt:P0AEK4

fabI392(ts)

C to T at position 1125

Ser to Phe at position 241

Growth Phenotype

temperature sensitive

PMID:4574419[6]

CGSC:7550

originally named envM392

sequence of mutation[3]

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW1281

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCGGTTTTCTTTCCGGTAAGCG

Primer 2:CCTTTCAGTTCGAGTTCGTTCAT

18B6

Kohara Phage

Genobase

PMID:3038334[8]

E14F6

Kohara Phage

Genobase

PMID:3038334[8]

trpC83::Tn10

Linked marker

CAG18455 = CGSC7371[9]

est. P1 cotransduction: 30% [10]
Synonyms:trpB83::Tn10

zcj-233::Tn10

Linked marker

CAG12028 = CGSC7373[9]

est. P1 cotransduction: 47% [10]
Synonyms:zci-233::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11528

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11528

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945870

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001482

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1490

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0004327

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 Bergler, H et al. (1992) Sequences of the envM gene and of two mutated alleles in Escherichia coli. J. Gen. Microbiol. 138 2093-100 PubMed
  4. ↑ Bergler, H et al. (1994) Protein EnvM is the NADH-dependent enoyl-ACP reductase (FabI) of Escherichia coli. J. Biol. Chem. 269 5493-6 PubMed
  5. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  6. ↑ Egan, AF & Russell, RR (1973) Conditional mutations affecting the cell envelope of Escherichia coli K-12. Genet. Res. 21 139-52 PubMed
  7. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. ↑ 8.0 8.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  9. ↑ 9.0 9.1 CGSC: The Coli Genetics Stock Center
  10. ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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