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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG11577 ECOCYC:EG11577]
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
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Escherichia coli str. K-12 substr. MG1655  
Escherichia coli str. K-12 substr. MG1655  
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RegulonDB:ECK120001529
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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EchoBASE:EB1537
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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ASAP:ABE-0000265
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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Revision as of 07:18, 27 May 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

leuB

Mnemonic

Leucine (biosynthesis)

Synonyms

ECK0075, b0073, JW5807[1], JW5807

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

1.74 minutes 

MG1655: 81958..80867
<gbrowseImage> name=NC_000913:80867..81958 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 84762..83671
<gbrowseImage> name=NC_012967:83671..84762 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 80866..81957
<gbrowseImage> name=NC_012759:80866..81957 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 81958..80867
<gbrowseImage> name=AC_000091:80867..81958 source=W3110 preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

80870

Edman degradation

PMID:9298646[2]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

leuB(del) (Keio:JW5807)

deletion

deletion

PMID:16738554[3]

Shigen

leuB6(Am)

PMID:6224549[4]

CGSC:4494

amber (UAG) mutation

leuB401

CGSC:6568

leuB61

CGSC:6570

leuB19

CGSC:8331


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5807

Plasmid clone

Shigen

PMID:16769691[5]

Status:Clone OK

Primer 1:GCCTCGAAGAATTACCATATTGC

Primer 2:CCaACCCCTTCTGCTACATAGCG

8H11

Kohara Phage

Genobase

PMID:3038334[6]

6C1

Kohara Phage

Genobase

PMID:3038334[6]

carB96::Tn10

Linked marker

CAG12093 = CGSC7322[7]

est. P1 cotransduction: 11% [8]
Synonyms:car-96::Tn10

leuO3051::Tn10

Linked marker

CAG12095 = CGSC7324[7]

est. P1 cotransduction: 92% [8]
Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG11577

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11577

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:944798

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001529

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1537

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000265

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  3. ↑ Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ Drapeau, GR et al. (1983) Unusual properties of a new division mutant of Escherichia coli. Can. J. Microbiol. 29 694-9 PubMed
  5. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  6. ↑ 6.0 6.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  7. ↑ 7.0 7.1 CGSC: The Coli Genetics Stock Center
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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