leuB:Gene Product(s)

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Nomenclature Function Interactions Localization Sequence Domains Structure Resources Accessions Links References Suggestions

Nomenclature

See Help:Product_nomenclature for help entering or editing information in this section of EcoliWiki.

Standard name

LeuB

Synonyms

3-isopropylmalate dehydrogenase[1], B0073[2][1], LeuB[2][1] , ECK0075, JW5807, b0073

Product description

LeuB[2][3];

Component of 3-isopropylmalate dehydrogenase[2][3]

beta-Isopropylmalate dehydrogenase[4]

EC number (for enzymes)
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Notes

Function

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<protect> Gene Ontology
See Help:Gene_ontology for help entering or editing GO terms and GO annotations in EcoliWiki.

Qualifier GO ID GO term name Reference Evidence Code with/from Aspect Notes Status
GO:0003862

3-isopropylmalate dehydrogenase activity

PMID:9003442[5]

IDA: Inferred from Direct Assay

F

complete

GO:0034198

cellular response to amino acid starvation

PMID:10220423[6] PMID:15133107[7]

IMP: Inferred from Mutant Phenotype

P

complete

GO:0000287

magnesium ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001804

F

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR019818

F

Seeded from EcoCyc (v14.0)

complete

GO:0000287

magnesium ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0460

F

Seeded from EcoCyc (v14.0)

complete

GO:0003862

3-isopropylmalate dehydrogenase activity

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01033

F

Seeded from EcoCyc (v14.0)

complete

GO:0003862

3-isopropylmalate dehydrogenase activity

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004429

F

Seeded from EcoCyc (v14.0)

complete

GO:0003862

3-isopropylmalate dehydrogenase activity

GOA:spec
GO_REF:0000003

IEA: Inferred from Electronic Annotation

EC:1.1.1.85

F

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01033

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004429

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0963

C

Seeded from EcoCyc (v14.0)

complete

GO:0005737

cytoplasm

GO_REF:0000023

IEA: Inferred from Electronic Annotation

SP_SL:SL-0086

C

Seeded from EcoCyc (v14.0)

complete

GO:0009098

leucine biosynthetic process

GOA:hamap
GO_REF:0000020

IEA: Inferred from Electronic Annotation

HAMAP:MF_01033

P

Seeded from EcoCyc (v14.0)

complete

GO:0009098

leucine biosynthetic process

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004429

P

Seeded from EcoCyc (v14.0)

complete

GO:0009098

leucine biosynthetic process

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0432

P

Seeded from EcoCyc (v14.0)

complete

GO:0030145

manganese ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0464

F

Seeded from EcoCyc (v14.0)

complete

GO:0046872

metal ion binding

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0479

F

Seeded from EcoCyc (v14.0)

complete

GO:0051287

NAD or NADH binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001804

F

Seeded from EcoCyc (v14.0)

complete

GO:0051287

NAD or NADH binding

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR019818

F

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR001804

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR004429

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:interpro
GO_REF:0000002

IEA: Inferred from Electronic Annotation

InterPro:IPR019818

P

Seeded from EcoCyc (v14.0)

complete

GO:0055114

oxidation reduction

GOA:spkw
SP_KW:GO_REF:0000004

IEA: Inferred from Electronic Annotation

SP_KW:KW-0560

P

Seeded from EcoCyc (v14.0)

complete

GO:0009098

leucine biosynthetic process

PMID:4570778[8]

IMP: Inferred from Mutant Phenotype

P

complete

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Interactions See Help:Product_interactions for help entering or editing information about gene product interactions in this section of EcoliWiki.

Partner Type Partner Notes References Evidence

Protein

Subunits of 3-isopropylmalate dehydrogenase

could be indirect


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Notes

Localization

See Help:Product_localization for how to add or edit information in this section of EcoliWiki.

Compartment Description Evidence Reference/Source Notes
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Notes

Structure and Physical Properties

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<protect> Physical Properties See Help:Product_physical_properties for help entering or editing information about the physical properties of this gene product.

Name
Sequence

at EcoCyc 

MMSKNYHIAV LPGDGIGPEV MTQALKVLDA VRNRFAMRIT TSHYDVGGAA IDNHGQPLPP
ATVEGCEQAD AVLFGSVGGP KWEHLPPDQQ PERGALLPLR KHFKLFSNLR PAKLYQGLEA
FCPLRADIAA NGFDILCVRE LTGGIYFGQP KGREGSGQYE KAFDTEVYHR FEIERIARIA
FESARKRRHK VTSIDKANVL QSSILWREIV NEIATEYPDV ELAHMYIDNA TMQLIKDPSQ
FDVLLCSNLF GDILSDECAM ITGSMGMLPS ASLNEQGFGL YEPAGGSAPD IAGKNIANPI
AQILSLALLL RYSLDADDAA CAIERAINRA LEEGIRTGDL ARGAAAVSTD EMGDIIARYV AEGV
Length

364

Mol. Wt

39.649 kDa

pI

5.0 (calculated)

Extinction coefficient

27,390 - 28,140 (calc based on 11 Y, 2 W, and 6 C residues)

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Domains/Motifs/Modification Sites

See Help:Product_domains_motifs for help entering or editing information in this section of EcoliWiki.

Type Residues Description Notes References

Initiator Methionine

1

Removed

UniProt:P30125

Domain

6..355

PF00180 Isocitrate/isopropylmalate dehydrogenase

PMID:19920124[9]

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Structure
See Help:Product_structure for help entering or editing information in this section of EcoliWiki.

Structures

<beststructure> gene=leuB taxon=562,83333 </beststructure>

Models

View models at:

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Structure figures

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Notes

Gene Product Resources

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See Help:Product_resources for help with entering or editing information in this section of EcoliWiki.

Resource type Source Notes/Reference
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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

NCBI (Protein) (EcoliWiki Page)

GI:90111082

Escherichia coli str. K-12 substr. MG1655

NCBI (Protein) (EcoliWiki Page)

GeneID:944798

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0000265

Escherichia coli str. K-12 substr. MG1655

UniProt (EcoliWiki Page)

UniProtKB/Swiss-Prot:P30125

Escherichia coli str. K-12 substr. MG1655

EcoCyc

EcoCyc:EG11577

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG11577

Escherichia coli str. K-12 substr. MG1655

NCBI (Gene) (EcoliWiki Page)

GeneID:944798

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120001529

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB1537

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ 1.0 1.1 1.2 1.3 Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 2.2 2.3 EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ 3.0 3.1 EcoCyc (release 11.1; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  4. ↑ EcoGene: Rudd, KE (2000) EcoGene: a genome sequence database for Escherichia coli K-12. Nucleic Acids Res 28:60-4.
  5. ↑ Wallon, G et al. (1997) Purification, catalytic properties and thermostability of 3-isopropylmalate dehydrogenase from Escherichia coli. Biochim. Biophys. Acta 1337 105-12 PubMed
  6. ↑ Wright, BE et al. (1999) Hypermutation in derepressed operons of Escherichia coli K12. Proc. Natl. Acad. Sci. U.S.A. 96 5089-94 PubMed
  7. ↑ Reimers, JM et al. (2004) Increased transcription rates correlate with increased reversion rates in leuB and argH Escherichia coli auxotrophs. Microbiology (Reading, Engl.) 150 1457-66 PubMed
  8. ↑ Somers, JM et al. (1973) Genetic fine structure of the leucine operon of Escherichia coli K-12. J. Bacteriol. 113 1268-72 PubMed
  9. ↑ Finn, RD et al. (2010) The Pfam protein families database. Nucleic Acids Res. 38 D211-22 PubMed

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