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!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10766 ECOCYC:EG10766]
EcoCyc:EG10766
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[EcoGene]]
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[http://ecogene.org/geneinfo.php?eg_id=EG10766 EcoGene:EG10766]
EcoGene:EG10766
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Escherichia coli str. K-12 substr. MG1655  
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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Escherichia coli str. K-12 substr. MG1655  
Escherichia coli str. K-12 substr. MG1655  
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[[RegulonDB]]
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RegulonDB:ECK120000757
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[EchoBASE]]
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EchoBASE:EB0759
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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[[ASAP]]
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ASAP:ABE-0010814
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''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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Revision as of 07:29, 27 May 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

secY

Mnemonic

Secretory

Synonyms

ECK3287, b3300, JW3262, prlA[1], prlA

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

74.16 minutes 

MG1655: 3442119..3440788
<gbrowseImage> name=NC_000913:3440788..3442119 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3370671..3372002
<gbrowseImage> name=NC_012967:3370671..3372002 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3327945..3329276
<gbrowseImage> name=NC_012759:3327945..3329276 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4196319..4197650
<gbrowseImage> name=AC_000091:4196319..4197650 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3539864..3538533
<gbrowseImage> name=NC_010473:3538533..3539864 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

secYI290T

I290T

In secY121; alterated signal sequence interaction

seeded from UniProt:P0AGA2

secYP40S

P40S

In secY100; temperature-sensitive

seeded from UniProt:P0AGA2

secYR357H

R357H

In secY39; cold-sensitive

seeded from UniProt:P0AGA2

secYF67C

F67C

In prlA3; alterated signal sequence interaction

seeded from UniProt:P0AGA2

secYG167E

G167E

In secY100; temperature-sensitive

seeded from UniProt:P0AGA2

secYG240D

G240D

In secY24; temperature-sensitive

seeded from UniProt:P0AGA2

secYS282R

S282R

In prlA401; alterated signal sequence interaction

seeded from UniProt:P0AGA2

secYF286Y

F286Y

In prlA4-1; alterated signal sequence interaction

seeded from UniProt:P0AGA2

secYP287L

P287L

In secY161; alterated signal sequence interaction

seeded from UniProt:P0AGA2

secYA363S

A363S

In secY40; cold-sensitive

seeded from UniProt:P0AGA2

secYI408N

I408N

In prlA4-2; alterated signal sequence interaction

seeded from UniProt:P0AGA2

secY*

secY24(ts)

temperature sensitive

PMID:7665473[2] PMID:8650200[3]

CGSC:19326

secY125

Growth Phenotype

cold-sensitive

PMID:10852867[4] PMID:8190079[5]

secY215 (ts)

Growth Phenotype

growth stops at 42C and sensitivity to temperature becomes more pronounced with increase in salt concentration

PMID:6339072[6]

previously known as ts215

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3262

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCGCTAAACAACCGGGATTAGA

Primer 2:CCTCGGCCGTAGCCTTTCAGGTT

E1H9

Kohara Phage

Genobase

PMID:3038334[8]

smg-3082::Tn10

Linked marker

CAG12071 = CGSC7444[9]

est. P1 cotransduction: 66% [10]
Synonyms:zhb-3082::Tn10, zhd-3082::Tn10

zhf-3084::Tn10

Linked marker

CAG18456 = CGSC7576[9]

est. P1 cotransduction: 4% [10]
Synonyms:zhe-3084::Tn10nnnCAG18456 also carries cysG0 (CGSC).

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10766

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10766

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:947799

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000757

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0759

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0010814

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Danese, PN et al. (1995) Multicopy suppression of cold-sensitive sec mutations in Escherichia coli. J. Bacteriol. 177 4969-73 PubMed
  3. ↑ Nouwen, N et al. (1996) prlA suppressors in Escherichia coli relieve the proton electrochemical gradient dependency of translocation of wild-type precursors. Proc. Natl. Acad. Sci. U.S.A. 93 5953-7 PubMed
  4. ↑ Matsumoto, G et al. (2000) A mutation in secY that causes enhanced SecA insertion and impaired late functions in protein translocation. J. Bacteriol. 182 3377-82 PubMed
  5. ↑ Taura, T et al. (1994) Genetic analysis of SecY: additional export-defective mutations and factors affecting their phenotypes. Mol. Gen. Genet. 243 261-9 PubMed
  6. ↑ Ito, K et al. (1983) A temperature-sensitive mutant of E. coli exhibiting slow processing of exported proteins. Cell 32 789-97 PubMed
  7. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. ↑ Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  9. ↑ 9.0 9.1 CGSC: The Coli Genetics Stock Center
  10. ↑ 10.0 10.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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