murE:Gene: Difference between revisions

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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=109 Genobase]
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=109 Genobase]
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PMID:3038334
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[[:Category:Kohara_Phage:6F3|6F3]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=110 Genobase]
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PMID:3038334  
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est. P1 cotransduction: 7% <ref name='LIB:Nichols_linked_Tn'/>  
est. P1 cotransduction: 7% <ref name='LIB:Nichols_linked_Tn'/>  
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[[:Category:Kohara_Phage:6F3|6F3]]
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Kohara Phage
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[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=110 Genobase]
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PMID:3038334


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!|Database!!Accession!!Notes
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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10621 EcoCyc:EG10621]
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[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
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[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0616 EchoBASE:EB0616]
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[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
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[http://chase.ou.edu/macro/searchlink.php?bnum=b0085 EcoliGenExpDB:b0085]
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[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
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[http://BioCyc.org/ECOL199310/new-image?object=C0103 ECOL199310CYC:C0103]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
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[http://ecogene.org/geneinfo.php?eg_id=EG10621 EcoGene:EG10621]
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[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
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[http://BioCyc.org/ECOO157/new-image?object=MURE ECOO157CYC:MURE]
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[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
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[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/475 CGSC:475]
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[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0000311&formSubmitReturn=1 ASAP:ABE-0000311]
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Revision as of 20:47, 21 September 2011

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

murE

Mnemonic

Murein

Synonyms

ECK0086, b0085, JW0083[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

2.01 minutes 

MG1655: 93166..94653
<gbrowseImage> name=NC_000913:93166..94653 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 95970..97457
<gbrowseImage> name=NC_012967:95970..97457 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 93165..94652
<gbrowseImage> name=NC_012759:93165..94652 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 93166..94653
<gbrowseImage> name=AC_000091:93166..94653 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 67270..68757
<gbrowseImage> name=NC_010473:67270..68757 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

93169

Edman degradation

PMID:2198024[2]
PMID:2269304[3]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

murEA495S

A495S

(in murE1)

Strain variation; seeded from UniProt:P22188

murEE344K

E344K

(in murE1)

Strain variation; seeded from UniProt:P22188

murE1

CGSC:9851


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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0083

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCGCAGATCGTAATTTGCGCGA

Primer 2:CCTGCAATCACCCCCAGCAGACG

6C1

Kohara Phage

Genobase

PMID:3038334[5]

6F3

Kohara Phage

Genobase

PMID:3038334[5]

leuO3051::Tn10

Linked marker

CAG12095 = CGSC7324[6]

est. P1 cotransduction: 72% [7]
Synonyms:zab-3051::Tn10 nnnThe start of leuO was reannotated by Riley et al. (2006).

zad-220::Tn10

Linked marker

CAG12025 = CGSC7326[6]

est. P1 cotransduction: 7% [7]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes
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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Michaud, C et al. (1990) Revised interpretation of the sequence containing the murE gene encoding the UDP-N-acetylmuramyl-tripeptide synthetase of Escherichia coli. Biochem. J. 269 277-8 PubMed
  3. ↑ Michaud, C et al. (1990) Over-production, purification and properties of the uridine-diphosphate-N-acetylmuramoyl-L-alanyl-D-glutamate: meso-2,6-diaminopimelate ligase from Escherichia coli. Eur. J. Biochem. 194 853-61 PubMed
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. ↑ 5.0 5.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  6. ↑ 6.0 6.1 CGSC: The Coli Genetics Stock Center
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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