metA:Gene: Difference between revisions

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[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
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[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10581 EcoCyc:EG10581]
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[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
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[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0576 EchoBASE:EB0576]
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[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
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[http://chase.ou.edu/macro/searchlink.php?bnum=b4013 EcoliGenExpDB:b4013]
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[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
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[http://BioCyc.org/ECOL199310/new-image?object=C4970 ECOL199310CYC:C4970]
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[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
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[http://ecogene.org/geneinfo.php?eg_id=EG10581 EcoGene:EG10581]
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[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
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[http://BioCyc.org/ECOO157/new-image?object=G7E-0 ECOO157CYC:G7E-0]
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[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
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[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/516 CGSC:516]
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[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
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[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0013121&formSubmitReturn=1 ASAP:ABE-0013121]
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Revision as of 20:41, 21 September 2011

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

metA

Mnemonic

Methionine

Synonyms

ECK4005, b4013, JW3973[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

90.79 minutes, 90.79 minutes 

MG1655: 4212303..4213232
<gbrowseImage> name=NC_000913:4212303..4213232 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4193725..4194654
<gbrowseImage> name=NC_012967:4193725..4194654 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4101983..4102912
<gbrowseImage> name=NC_012759:4101983..4102912 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4217870..4218799
<gbrowseImage> name=AC_000091:4217870..4218799 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4311999..4312928
<gbrowseImage> name=NC_010473:4311999..4312928 source=DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes


Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔmetA (Keio:JW3973)

deletion

deletion

PMID:16738554[2]

Shigen
CGSC10856[3]

metA28

CGSC:7080

metA115

CGSC:8439

metA160

CGSC:10344

ΔmetA780::kan

PMID:16738554[2]

CGSC:101824



Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3973

Plasmid clone

Shigen

PMID:16769691[4]

Status:Clone OK

Primer 1:GCCCCGATTCGTGTGCCGGACGA

Primer 2:CCATCCAGCGTTGGATTCATGTG

thiC39::Tn10

Linked marker

CAG18500 = CGSC7472[3]

est. P1 cotransduction: 52% [5]
Synonyms:thi-39::Tn10

malF3089::Tn10

Linked marker

CAG12164 = CGSC7476[3]

est. P1 cotransduction: 33% [5]


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes


Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ 2.0 2.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  3. ↑ 3.0 3.1 3.2 CGSC: The Coli Genetics Stock Center
  4. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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