dppA:Gene: Difference between revisions

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Revision as of 19:55, 17 August 2011

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dppA

Mnemonic

Dipeptide permease system

Synonyms

ECK3531, b3544, JW3513, alu, dpp, fpp[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

79.84 minutes 

MG1655: 3705728..3704121
<gbrowseImage> name=NC_000913:3704121..3705728 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3638163..3636556
<gbrowseImage> name=NC_012967:3636556..3638163 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3592615..3594222
<gbrowseImage> name=NC_012759:3592615..3594222 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3932710..3934317
<gbrowseImage> name=AC_000091:3932710..3934317 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3803473..3801866
<gbrowseImage> name=NC_010473:3801866..3803473 source=DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3704124

Edman degradation

PMID:1956284[2]
PMID:9298646[3]
PMID:9600841[4]
PMID:9740056[5]
PMID:10972807[6]



Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔdppA (Keio:JW3513)

deletion

deletion

PMID:16738554[7]

Shigen
CGSC10599[8]

dppA::Tn5KAN-I-SceI (FB21271)

Insertion at nt 837 in Minus orientation

PMID:15262929[9]

E. coli Genome Project:FB21271

does not contain pKD46

ΔdppA728::kan

PMID:16738554[7]

CGSC:100636



Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3513

Plasmid clone

Shigen

PMID:16769691[10]

Status:Clone OK

Primer 1:GCCCGTATTTCCTTGAAAAAGTC

Primer 2:CCTTCGATAGAGACGTTTTCGAA

zhg-50::Tn10

Linked marker

CAG18450 = CGSC7450[8]

est. P1 cotransduction: % [11]
Synonyms:zhf-50::Tn10, zhf-5::Tn10

zic-4901::Tn10

Linked marker

CAG18492 = CGSC7457[8]

est. P1 cotransduction: % [11]


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10248

EchoBASE (EcoliWiki Page)

EchoBASE:EB0244

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b3544

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C4361

EcoGene (EcoliWiki Page)

EcoGene:EG10248

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:DPPA

CGSC (EcoliWiki Page)

CGSC:35111

ASAP (EcoliWikiPage)

ASAP:ABE-0011576



Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Abouhamad, WN et al. (1991) Peptide transport and chemotaxis in Escherichia coli and Salmonella typhimurium: characterization of the dipeptide permease (Dpp) and the dipeptide-binding protein. Mol. Microbiol. 5 1035-47 PubMed
  3. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  4. ↑ Wilkins, MR et al. (1998) Protein identification with N and C-terminal sequence tags in proteome projects. J. Mol. Biol. 278 599-608 PubMed
  5. ↑ Tonella, L et al. (1998) '98 Escherichia coli SWISS-2DPAGE database update. Electrophoresis 19 1960-71 PubMed
  6. ↑ Urbanowski, ML et al. (2000) The gcvB gene encodes a small untranslated RNA involved in expression of the dipeptide and oligopeptide transport systems in Escherichia coli. Mol. Microbiol. 37 856-68 PubMed
  7. ↑ 7.0 7.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  8. ↑ 8.0 8.1 8.2 CGSC: The Coli Genetics Stock Center
  9. ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  10. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  11. ↑ 11.0 11.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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