rmf:Gene: Difference between revisions

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Revision as of 20:21, 17 August 2011

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

rmf

Mnemonic

Ribosome modulation factor

Synonyms

ECK0944, b0953, JW0936, ramB, rimF, rimG, res[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

21.88 minutes 

MG1655: 1014938..1015105
<gbrowseImage> name=NC_000913:1014938..1015105 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1032806..1032973
<gbrowseImage> name=NC_012967:1032806..1032973 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 917906..918073
<gbrowseImage> name=NC_012759:917906..918073 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1016137..1016304
<gbrowseImage> name=AC_000091:1016137..1016304 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1068866..1069033
<gbrowseImage> name=NC_010473:1068866..1069033 source=DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1014938

Edman degradation

PMID:2181444[2]



Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δrmf (Keio:JW0936)

deletion

deletion

PMID:16738554[3]

Shigen
CGSC8940[4]

rmf::Tn5KAN-2 (FB20071)

Insertion at nt 45 in Plus orientation

PMID:15262929[5]

E. coli Genome Project:FB20071

does not contain pKD46

rimG21

CGSC:10347

rimG22

CGSC:11197

Δrmf-769::kan

PMID:16738554[3]

CGSC:102738



Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0936

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCAAGAGACAAAAACGAGATCG

Primer 2:CCGGCCATTACTACCCTGTCCGC

zcb-3059::Tn10

Linked marker

CAG12094 = CGSC7358[4]

est. P1 cotransduction: 64% [7]

zcc-282::Tn10

Linked marker

CAG18466 = CGSC7359[4]

est. P1 cotransduction: 12% [7]


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG50004

EchoBASE (EcoliWiki Page)

EchoBASE:EB4298

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b0953

EcoGene (EcoliWiki Page)

EcoGene:EG50004

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:RMF

CGSC (EcoliWiki Page)

CGSC:31760

ASAP (EcoliWikiPage)

ASAP:ABE-0003227



Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Wada, A et al. (1990) Structure and probable genetic location of a "ribosome modulation factor" associated with 100S ribosomes in stationary-phase Escherichia coli cells. Proc. Natl. Acad. Sci. U.S.A. 87 2657-61 PubMed
  3. ↑ 3.0 3.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  4. ↑ 4.0 4.1 4.2 CGSC: The Coli Genetics Stock Center
  5. ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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