ubiC:Gene: Difference between revisions

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source=MG1655_W3110
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Revision as of 17:49, 27 June 2011

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

ubiC

Mnemonic

Ubiquinone

Synonyms

ECK4031, b4039, JW5713, sdgG?[1][2]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

91.61 minutes 

MG1655: 4250529..4251026
<gbrowseImage> name=NC_000913:4250529..4251026 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4231438..4231935
<gbrowseImage> name=NC_012967:4231438..4231935 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4189374..4189871
<gbrowseImage> name=NC_012759:4189374..4189871 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4256096..4256593
<gbrowseImage> name=AC_000091:4256096..4256593 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4350225..4350722
<gbrowseImage> name=NC_010473:4350225..4350722 source=DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4250532

Edman degradation

PMID:1644758[3]



Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

ΔubiC (Keio:JW5713)

deletion

deletion

PMID:16738554[4]

Shigen
CGSC11535[5]

ubiCG91A

G91A

Increases the inhibition by product by about 40%. No effect on substrate affinity

seeded from UniProt:P26602

ubiCE156K

E156K

Loss of activity

seeded from UniProt:P26602

ΔubiC736::kan

PMID:16738554[4]

CGSC:103268



Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW5713

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCTCACACCCCGCGTTAACGCA

Primer 2:CCGTACAACGGTGACGCCGGTAA

malF3089::Tn10

Linked marker

CAG12164 = CGSC7476[5]

est. P1 cotransduction: 71% [7]

zje-2241::Tn10

Linked marker

CAG18427 = CGSC7481[5]

est. P1 cotransduction: 7% [7]


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG11369

EchoBASE (EcoliWiki Page)

EchoBASE:EB1343

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b4039

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C5009

EcoGene (EcoliWiki Page)

EcoGene:EG11369

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:UBIC

CGSC (EcoliWiki Page)

CGSC:48

ASAP (EcoliWikiPage)

ASAP:ABE-0013227



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ EcoCyc (release 10.6; 2007) Keseler, IM et al. (2005) Nucleic Acids Res. 33(Database issue):D334-7
  3. ↑ Nichols, BP & Green, JM (1992) Cloning and sequencing of Escherichia coli ubiC and purification of chorismate lyase. J. Bacteriol. 174 5309-16 PubMed
  4. ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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