grpE:Gene: Difference between revisions

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Revision as of 20:55, 26 July 2010

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

grpE

Mnemonic

Growth after phage induction

Synonyms

ECK2610, b2614, JW2594[1]

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

59.23 minutes 

MG1655: 2748730..2748137
<gbrowseImage> name=NC_000913:2748137..2748730 source=ecoli preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 2672003..2671410
<gbrowseImage> name=NC_012967:2671410..2672003 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 2633949..2634542
<gbrowseImage> name=NC_012759:2633949..2634542 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 2749364..2748771
<gbrowseImage> name=AC_000091:2748771..2749364 source=ecoli_W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 2840495..2839902
<gbrowseImage> name=NC_010473:2839902..2840495 source=ecoli_DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes
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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

grpEV108A

V108A

No effect in ability to interact with dnaK

seeded from UniProt:P09372

grpEG122D

G122D

Temperature-sensitive phenotype

seeded from UniProt:P09372

grpEL149A

L149A

No effect in ability to interact with dnaK

seeded from UniProt:P09372

grpEQ155A

Q155A

No effect in ability to interact with dnaK

seeded from UniProt:P09372

grpEI157A

I157A

No effect in ability to interact with dnaK

seeded from UniProt:P09372

grpEM159A

M159A

No effect in ability to interact with dnaK

seeded from UniProt:P09372

grpEM174A

M174A

No effect in ability to interact with dnaK

seeded from UniProt:P09372

grpER183A

R183A

Loss of ability to interact with dnaK

seeded from UniProt:P09372

grpER186A

R186A

No effect in ability to interact with dnaK

seeded from UniProt:P09372

grpEM189A

M189A

No effect in ability to interact with dnaK

seeded from UniProt:P09372

grpEV192A

V192A

Loss of ability to interact with dnaK

seeded from UniProt:P09372

grpER74A

R74A

Great decrease in ability to interact with dnaK

seeded from UniProt:P09372

grpEP151A

P151A

No effect in ability to interact with dnaK

seeded from UniProt:P09372

grpER73A

R73A

Great decrease in ability to interact with dnaK

seeded from UniProt:P09372

grpEK82A

K82A

Great decrease in ability to interact with dnaK

seeded from UniProt:P09372

grpEF86A

F86A

No effect in ability to interact with dnaK

seeded from UniProt:P09372

grpER104A

R104A

No effect in ability to interact with dnaK

seeded from UniProt:P09372

grpEE107A

E107A

No effect in ability to interact with dnaK

seeded from UniProt:P09372

grpE280

PMID:2424889[2]

CGSC:90840


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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW2594

Plasmid clone

Shigen

PMID:16769691[3]

Status:Clone OK

Primer 1:GCCAGTAGTAAAGAACAGAAAAC

Primer 2:CCAGCTTTTGCTTTCGCTACAG

pheA18::Tn10

Linked marker

CAG12158 = CGSC7421[4]

est. P1 cotransduction: 69% [5]

srlD3131::Tn10

Linked marker

CAG18642 = CGSC7423[4]

est. P1 cotransduction: % [5]
Synonyms:zfh-3131::Tn10, zfi-3131::Tn10

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG10416

EchoBASE (EcoliWiki Page)

EchoBASE:EB0411

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b2614

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C3135

EcoGene (EcoliWiki Page)

EcoGene:EG10416

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:GRPE

CGSC (EcoliWiki Page)

CGSC:660

ASAP (EcoliWikiPage)

ASAP:ABE-0008598


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Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Ang, D et al. (1986) Escherichia coli grpE gene codes for heat shock protein B25.3, essential for both lambda DNA replication at all temperatures and host growth at high temperature. J. Bacteriol. 167 25-9 PubMed
  3. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  4. ↑ 4.0 4.1 CGSC: The Coli Genetics Stock Center
  5. ↑ 5.0 5.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

Categories

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