efp:Gene: Difference between revisions

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Revision as of 20:38, 26 July 2010

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

efp

Mnemonic

Elongation Factor-Peptidyltransferase

Synonyms

ECK4141, b4147, JW4107[1]


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

94.27 minutes 

MG1655: 4373722..4374288
<gbrowseImage> name=NC_000913:4373722..4374288 source=ecoli preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4354410..4354976
<gbrowseImage> name=NC_012967:4354410..4354976 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4312457..4313023
<gbrowseImage> name=NC_012759:4312457..4313023 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4380379..4380945
<gbrowseImage> name=AC_000091:4380379..4380945 source=ecoli_W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4474084..4474650
<gbrowseImage> name=NC_010473:4474084..4474650 source=ecoli_DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4373725

Edman degradation

PMID:1956781[2]
PMID:9298646[3]



Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δefp (Keio:JW4107)

deletion

deletion

PMID:16738554[4]

Shigen
CGSC10957[5]

Δefp-772::kan

PMID:16738554[4]

CGSC:100688



Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4107

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCGCAACGTACTATAGCAACGA

Primer 2:CCtTTCACGCGAGAGACGTATTC

cadB2231::Tn10

Linked marker

CAG18488 = CGSC7479[5]

est. P1 cotransduction: 54% [7]
Synonyms:zjd-2231::Tn10

cycA30::Tn10

Linked marker

CAG12073 = CGSC7482[5]

est. P1 cotransduction: 8% [7]


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc (EcoliWiki Page)

EcoCyc:EG12099

EchoBASE (EcoliWiki Page)

EchoBASE:EB2023

EcoliGenExpDB (EcoliWiki Page)

EcoliGenExpDB:b4147

ECOL199310CYC (EcoliWiki Page)

ECOL199310CYC:C5232

EcoGene (EcoliWiki Page)

EcoGene:EG12099

ECOO157CYC (EcoliWiki Page)

ECOO157CYC:EFP

CGSC (EcoliWiki Page)

CGSC:34470

ASAP (EcoliWikiPage)

ASAP:ABE-0013583



Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Aoki, H et al. (1991) Cloning, sequencing and overexpression of the gene for prokaryotic factor EF-P involved in peptide bond synthesis. Nucleic Acids Res. 19 6215-20 PubMed
  3. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  4. ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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