tsf:Gene: Difference between revisions
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Coding Start (SO:0000323) | |||
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[[MG1655]] | |||
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190860 | |||
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Edman degradation | |||
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PMID:8740179<br />PMID:9298646<br />PMID:9600841<br />PMID:9740056<br /> | |||
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Revision as of 19:03, 11 May 2010
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| Quickview | Gene | Gene Product(s) | Expression | Evolution | On One Page |
| Nomenclature | Location(s) and DNA Sequence | Sequence Features | Alleles and Phenotypes | Genetic Resources | Accessions | Links | References | Suggestions |
Nomenclature
See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.
| Standard name |
tsf |
|---|---|
| Mnemonic |
Translation stable factor |
| Synonyms |
ECK0169, b0170, JW0165[1] |
| edit table |
Notes
Location(s) and DNA Sequence
See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.
| Strain | Map location | Genome coordinates | Genome browsers | Sequence links |
|---|---|---|---|---|
|
MG1655 |
4.11 minutes |
MG1655: 190857..191708 |
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NC_012967: 193699..194550 |
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NC_012759: 190856..191707 |
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W3110 |
|
W3110: 190857..191708 |
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|
DH10B: 164961..165812 |
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| edit table |
Notes
Sequence Features
See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.
| Feature Type | Strain | Genomic Location | Evidence | Reference | Notes |
|---|---|---|---|---|---|
|
Coding Start (SO:0000323) |
190860 |
Edman degradation |
PMID:8740179[2] |
| |
| edit table |
Notes
Alleles and Phenotypes
See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.
| Allele | Nt change(s) | AA change(s) | Phenotype: Type | Phenotype: Description | Reference | Availability | Comments |
|---|---|---|---|---|---|---|---|
|
tsfF82A |
F82A |
2 to 3-fold less active in promoting GDP exchange and 6-fold less active in binding to EF-Tu |
seeded from UniProt:P0A6P1 | ||||
|
tsfH148A |
H148A |
At least 100-fold decrease in affinity between EF-Ts and EF-Tu and only small amount of GDP exchange activity |
seeded from UniProt:P0A6P1 | ||||
|
tsfK24A |
K24A |
No change in binding to EF-Tu and in promoting GDP exchange |
seeded from UniProt:P0A6P1 | ||||
|
tsfD81A |
D81A |
2 to 3-fold less active in promoting GDP exchange and slightly lower binding constant for interaction with EF- Tu |
seeded from UniProt:P0A6P1 | ||||
| edit table |
Notes
Genetic Resources
See Help:Gene_resources for help entering information into the Genetic Resources table.
| Resource | Resource Type | Source | Notes/Reference |
|---|---|---|---|
|
JW0165 |
Plasmid clone |
Status:Clone OK Primer 1:GCCGCTGAAATTACCGCATCCCT Primer 2:CCAGACTGCTTGGACATCGCAGC | |
|
Linked marker |
est. P1 cotransduction: 16% [8] | ||
|
Linked marker |
est. P1 cotransduction: 17% [8] | ||
| edit table |
Notes
Accessions in Other Databases
See Help:Gene_accessions for help with entering information into the Gene Accessions table.
| Database | Accession | Notes |
|---|---|---|
|
| ||
| edit table |
Notes
Links
| Name | URL | Comments |
|---|---|---|
| edit table |
<protect>
References
See Help:References for how to manage references in EcoliWiki.
- ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
- ↑ Pasquali, C et al. (1996) Two-dimensional gel electrophoresis of Escherichia coli homogenates: the Escherichia coli SWISS-2DPAGE database. Electrophoresis 17 547-55 PubMed
- ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
- ↑ Wilkins, MR et al. (1998) Protein identification with N and C-terminal sequence tags in proteome projects. J. Mol. Biol. 278 599-608 PubMed
- ↑ Tonella, L et al. (1998) '98 Escherichia coli SWISS-2DPAGE database update. Electrophoresis 19 1960-71 PubMed
- ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
- ↑ 7.0 7.1 CGSC: The Coli Genetics Stock Center
- ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).