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|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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****************************************************************************************** -->{{Gene_nomenclature_help}}
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{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  
{|   id="T46a9bd7b0f211" class=" tableEdit Gene_nomenclature_table"


|-
|-
!align=left {{table_heading_style}} |Standard name
!align=left {{table_heading_style}} |Standard name
||
||
''agp''  
''agp''
|-
|-
!align=left {{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Mnemonic
||
||
 
Acid glucose-1-phosphatase
|-
|-
!align=left {{table_heading_style}} |Synonyms
!align=left {{table_heading_style}} |Synonyms
||
||
''ECK0993, b1002, JW0987''<ref name='LIB:Riley_2006'/>
''ECK0993, b1002, JW0987''<ref name='LIB:Riley_2006'/>, JW0987''


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*  
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****************************************************************************************** -->{{Gene_location_help}}
****************************************************************************************** -->{{Gene_location_help}}
{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="U46a9bd7b8c5f2"  class=" tableEdit Gene_location_table"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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22.95 minutes&nbsp;
22.95 minutes&nbsp;
|
|
MG1655: 1064808..1066049<br />{{GBrowseFigureByCoord|1063808|1067049|ecoli|MG1655}}
MG1655: 1064808..1066049<br /><gbrowseImage>
name=NC_000913:1064808..1066049
source=MG1655
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10033 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10033 EcoCyc]
{{GBrowseLink|ecoli|NC_000913|1063808|1067049|GBrowse(MG1655)}}
{{GBrowseLink|MG1655|NC_000913|1063808|1067049|GBrowse(MG1655)}}
   
   
|
|
{{GBrowseFastaLink|ecoli|NC_000913|1064808|1066049|FASTA Dump}}
{{GBrowseFastaLink|MG1655|NC_000913|1064808|1066049|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG10033 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG10033 EcoCyc Sequence Dump]  
|-
|
[[REL606]]
|
|
NC_012967: 1081868..1083109<br /><gbrowseImage>
name=NC_012967:1081868..1083109
source=REL606
preset=GeneLocation
</gbrowseImage>
|
{{GBrowseLink|REL606|NC_012967|1081868|1083109|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|1081868|1083109|FASTA Dump}}
|-
|
[[BW2952]]
|
|
NC_012759: 967776..969017<br /><gbrowseImage>
name=NC_012759:967776..969017
source=BW2952
preset=GeneLocation
</gbrowseImage>
|
{{GBrowseLink|BW2952|NC_012759|967776|969017|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|967776|969017|FASTA Dump}}
|-  
|-  
|
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&nbsp;
&nbsp;
|
|
W3110: 1066007..1067248<br />{{GBrowseFigureByCoord|1065007|1068248|ecoli_W3110|W3110}}  
W3110: 1066007..1067248<br /><gbrowseImage>
name=NC_007779:1066007..1067248
source=W3110
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|W3110|NC_007779|1065007|1068248|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|NC_007779|1066007|1067248|FASTA Dump}}  
|-
|
[[DH10B]]
|
 
|
|
{{GBrowseLink|ecoli_W3110|AC_000091|1065007|1068248|GBrowse(W3110)}}
DH10B: 1118736..1119977<br /><gbrowseImage>
name=NC_010473:1118736..1119977
source=DH10B
preset=GeneLocation
</gbrowseImage>
 
|
|
{{GBrowseFastaLink|ecoli_W3110|AC_000091|1066007|1067248|FASTA Dump}}  
{{GBrowseLink|DH10B|NC_010473|1116254|1122459|GBrowse(DH10B)}}
|
{{GBrowseFastaLink|DH10B|NC_010473|1116254|1122459|FASTA Dump}}  


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*  
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****************************************************************************************** -->{{Gene_sequence_features_help}}
****************************************************************************************** -->{{Gene_sequence_features_help}}
{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="U46a9bd7c385f5"  class=" tableEdit Gene_sequence_features_table"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-
|
Coding Start (SO:0000323)
|
[[MG1655]]
|
1064874
|
Edman degradation
|
PMID:2153660<br />PMID:9298646<br />
|


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*  
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****************************************************************************************** -->{{Gene_alleles_help}}
****************************************************************************************** -->{{Gene_alleles_help}}
{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" {{Prettytable}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="Q46a9bd7c79115"  class=" tableEdit Gene_allele_table dataTable" 
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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|-  
|-  
|
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0003388 agp]
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0003388 agp::Tn5KAN-2] (FB20084)
|
|
 
Insertion at nt 327 in Minus orientation
|
|


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|
|
PMID:12519969
PMID:15262929
|
|
[[asapstrains:ASAP|ASAP]]
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB20084]
|
|
At position 327 in Minus orientation, does not contain plasmid  [[pKD46]].<br />ASAP strain name: FB20084
does not contain [[pKD46]]  
|-  
|-  
|
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===Notes===
===Notes===


==Genetic Interactions==
{{BacktoTop}}
<protect><!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.12563.M4e04b715ddd76-->
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="M4e04b715ddd76"  class=" tableEdit Genetic_interaction_table dataTable" 
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!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
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<!-- Genetic Interacdtions Notes -->
===Notes===


==Genetic Resources==
==Genetic Resources==
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|- {{table_heading_style}}
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!|Resource!!Resource Type!!Source!!Notes/Reference
!|Resource!!Resource Type!!Source!!Notes/Reference
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Primer 2:CCTTTCACCGCTTCATTCAACAC  
Primer 2:CCTTTCACCGCTTCATTCAACAC  
|-
|
[[:Category:Kohara_Phage:5A12|5A12]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=227 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:7B5|7B5]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=228 Genobase]
|
PMID:3038334
|-  
|-  
|
|
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est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/>  
est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/>  


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|- {{table_heading_style}}
|- {{table_heading_style}}
!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-  
|-  
|
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
|
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10033 EcoCyc:EG10033]
EcoCyc:EG10033
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
[[EcoGene]]
|
|
[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0032 EchoBASE:EB0032]
EcoGene:EG10033
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://chase.ou.edu/macro/searchlink.php?bnum=b1002 EcoliGenExpDB:b1002]
RegulonDB:ECK120000030
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
|
[http://BioCyc.org/ECOL199310/new-image?object=C1137 ECOL199310CYC:C1137]
[http://www.ncbi.nlm.nih.gov/gene?term=945773 GeneID:945773]
|
|
Escherichia coli str. K-12 substr. MG1655
|-  
|-  
|
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://ecogene.org/geneinfo.php?eg_id=EG10033 EcoGene:EG10033]
EchoBASE:EB0032
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
[[ASAP]]
|
|
[http://BioCyc.org/ECOO157/new-image?object=AGP ECOO157CYC:AGP]
ASAP:ABE-0003388
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]  
|-  
|
[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
|
[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/31830 CGSC:31830]
|
|-
|
[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0003388&formSubmitReturn=1 ASAP:ABE-0003388]
|


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==References==
==References==
{{BacktoTop}}
{{BacktoTop}}
 
{{RefHelp}}
<references/>
<references />
__NOTOC__
__NOTOC__
</protect></noinclude>
===Categories===
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:MG1655]]
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[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:agp genes]][[Category:Gene_List:DH10B]]
[[Category:agp genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

Latest revision as of 20:31, 19 September 2013

{{#css:Suppresslinks.css}}<css>h1 .editsection { display:none;} h2 .editsection { display:none;}</css>

Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

agp

Mnemonic

Acid glucose-1-phosphatase

Synonyms

ECK0993, b1002, JW0987[1], JW0987


Notes

Location(s) and DNA Sequence

[back to top]


See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

22.95 minutes 

MG1655: 1064808..1066049
<gbrowseImage> name=NC_000913:1064808..1066049 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 1081868..1083109
<gbrowseImage> name=NC_012967:1081868..1083109 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 967776..969017
<gbrowseImage> name=NC_012759:967776..969017 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 1066007..1067248
<gbrowseImage> name=NC_007779:1066007..1067248 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 1118736..1119977
<gbrowseImage> name=NC_010473:1118736..1119977 source=DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

[back to top]


See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

1064874

Edman degradation

PMID:2153660[2]
PMID:9298646[3]



Notes

Alleles and Phenotypes

[back to top]


See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δagp (Keio:JW0987)

deletion

deletion

PMID:16738554[4]

Shigen
CGSC8974[5]

agp::Tn5KAN-2 (FB20084)

Insertion at nt 327 in Minus orientation

PMID:15262929[6]

E. coli Genome Project:FB20084

does not contain pKD46

Δagp-746::kan

PMID:16738554[4]

CGSC:99952



Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW0987

Plasmid clone

Shigen

PMID:16769691[7]

Status:Clone OK

Primer 1:GCCAACAAAACGCTAATCGCCGC

Primer 2:CCTTTCACCGCTTCATTCAACAC

5A12

Kohara Phage

Genobase

PMID:3038334[8]

7B5

Kohara Phage

Genobase

PMID:3038334[8]

zcc-282::Tn10

Linked marker

CAG18466 = CGSC7359[5]

est. P1 cotransduction: 93% [9]

zce-726::Tn10

Linked marker

CAG12078 = CGSC7361[5]

est. P1 cotransduction: % [9]


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10033

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10033

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000030

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:945773

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0032

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0003388

Escherichia coli str. K-12 substr. MG1655


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Pradel, E et al. (1990) Nucleotide sequence and transcriptional analysis of the Escherichia coli agp gene encoding periplasmic acid glucose-1-phosphatase. J. Bacteriol. 172 802-7 PubMed
  3. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  4. ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  7. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  8. ↑ 8.0 8.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  9. ↑ 9.0 9.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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