efp:Gene: Difference between revisions

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|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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*  
*  
****************************************************************************************** -->{{Gene_nomenclature_help}}
****************************************************************************************** -->{{Gene_nomenclature_help}}
{|border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  
{|   id="T46aa084dcf783" class=" tableEdit Gene_nomenclature_table"


|-
|-
!align=left {{table_heading_style}} |Standard name
!align=left {{table_heading_style}} |Standard name
||
||
''efp''  
''efp''
|-
|-
!align=left {{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Mnemonic
||
||
 
Elongation Factor-Peptidyltransferase
|-
|-
!align=left {{table_heading_style}} |Synonyms
!align=left {{table_heading_style}} |Synonyms
||
||
''ECK4141, b4147, JW4107''<ref name='LIB:Riley_2006'/>
''ECK4141, b4147, JW4107''<ref name='LIB:Riley_2006'/>, JW4107''


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*  
*  
****************************************************************************************** -->{{Gene_location_help}}
****************************************************************************************** -->{{Gene_location_help}}
{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="K46aa084e5ed05"  class=" tableEdit Gene_location_table"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
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94.27 minutes&nbsp;
94.27 minutes&nbsp;
|
|
MG1655: 4373722..4374288<br />{{GBrowseFigureByCoord|4372722|4375288|ecoli|MG1655}}
MG1655: 4373722..4374288<br /><gbrowseImage>
name=NC_000913:4373722..4374288
source=MG1655
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG12099 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG12099 EcoCyc]
{{GBrowseLink|ecoli|NC_000913|4372722|4375288|GBrowse(MG1655)}}
{{GBrowseLink|MG1655|NC_000913|4372722|4375288|GBrowse(MG1655)}}
   
   
|
|
{{GBrowseFastaLink|ecoli|NC_000913|4373722|4374288|FASTA Dump}}
{{GBrowseFastaLink|MG1655|NC_000913|4373722|4374288|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG12099 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG12099 EcoCyc Sequence Dump]  
|-
|
[[REL606]]
|
|
NC_012967: 4354410..4354976<br /><gbrowseImage>
name=NC_012967:4354410..4354976
source=REL606
preset=GeneLocation
</gbrowseImage>
|
{{GBrowseLink|REL606|NC_012967|4354410|4354976|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|4354410|4354976|FASTA Dump}}
|-
|
[[BW2952]]
|
|
NC_012759: 4312457..4313023<br /><gbrowseImage>
name=NC_012759:4312457..4313023
source=BW2952
preset=GeneLocation
</gbrowseImage>
|
{{GBrowseLink|BW2952|NC_012759|4312457|4313023|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|4312457|4313023|FASTA Dump}}
|-  
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&nbsp;
&nbsp;
|
|
W3110: 4380379..4380945<br />{{GBrowseFigureByCoord|4379379|4381945|ecoli_W3110|W3110}}  
W3110: 4380379..4380945<br /><gbrowseImage>
name=NC_007779:4380379..4380945
source=W3110
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|W3110|NC_007779|4379379|4381945|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|NC_007779|4380379|4380945|FASTA Dump}}  
|-
|
[[DH10B]]
|
 
|
|
{{GBrowseLink|ecoli_W3110|AC_000091|4379379|4381945|GBrowse(W3110)}}
DH10B: 4474084..4474650<br /><gbrowseImage>
name=NC_010473:4474084..4474650
source=DH10B
preset=GeneLocation
</gbrowseImage>
 
|
|
{{GBrowseFastaLink|ecoli_W3110|AC_000091|4380379|4380945|FASTA Dump}}  
{{GBrowseLink|DH10B|NC_010473|4472952|4475782|GBrowse(DH10B)}}
|
{{GBrowseFastaLink|DH10B|NC_010473|4472952|4475782|FASTA Dump}}  


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*  
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****************************************************************************************** -->{{Gene_sequence_features_help}}
****************************************************************************************** -->{{Gene_sequence_features_help}}
{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="K46aa084f26be8"  class=" tableEdit Gene_sequence_features_table"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-
|
Coding Start (SO:0000323)
|
[[MG1655]]
|
4373725
|
Edman degradation
|
PMID:1956781<br />PMID:9298646<br />
|


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*  
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****************************************************************************************** -->{{Gene_alleles_help}}
****************************************************************************************** -->{{Gene_alleles_help}}
{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" {{Prettytable}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="D46aa084f72235"  class=" tableEdit Gene_allele_table dataTable" 
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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|
|


|[[PMID:16738554]]<ref name=PMID:16738554/>
|
PMID:16738554
|
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=109238 CGSC10957]<ref name='LIB:cgsc'/>  
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=109238 CGSC10957]<ref name='LIB:cgsc'/>  
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|[[PMID:16738554]]<ref name=PMID:16738554/>
|
PMID:16738554
|
|
[[cgscmutation:100688|CGSC:100688]]
[[cgscmutation:100688|CGSC:100688]]
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===Notes===
===Notes===


==Genetic Interactions==
{{BacktoTop}}
<protect><!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.18855.X4e04ba46d9c8a-->
<!--
******************************************************************************************
*
*  ** PLEASE DON'T EDIT THIS TABLE DIRECTLY.  Use the edit table link under the table. **
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="X4e04ba46d9c8a"  class=" tableEdit Genetic_interaction_table dataTable" 
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
|- class="tableEdit_footer"
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=bc145ea147f7e36caf09c1bfeed4ee9d.18855.X4e04ba46d9c8a&page=18855&pagename={{FULLPAGENAMEE}}&type=0&template=Genetic_interaction_table edit table]</span> || || || || || ||
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<!-- Genetic Interacdtions Notes -->
===Notes===


==Genetic Resources==
==Genetic Resources==
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|- {{table_heading_style}}
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!|Resource!!Resource Type!!Source!!Notes/Reference
!|Resource!!Resource Type!!Source!!Notes/Reference
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|
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|[[PMID:16769691]]<ref name=PMID:16769691/>
|
PMID:16769691


Status:Clone OK
Status:Clone OK
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Primer 2:CCtTTCACGCGAGAGACGTATTC  
Primer 2:CCtTTCACGCGAGAGACGTATTC  
|-
|
[[:Category:Kohara_Phage:9B1|9B1]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=649 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:1D4|1D4]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=650 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:3H6|3H6]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=651 Genobase]
|
PMID:3038334
|-  
|-  
|
|
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CAG18488 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40596 CGSC7479]<ref name='LIB:cgsc'/>
CAG18488 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40596 CGSC7479]<ref name='LIB:cgsc'/>
|
|
est. P1 cotransduction: 54% <ref name='LIB:Nichols_linked_Tn'/>nnSynonyms:zjd-2231::Tn10  
est. P1 cotransduction: 54% <ref name='LIB:Nichols_linked_Tn'/> <br />
Synonyms:zjd-2231::Tn10  
|-  
|-  
|
|
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est. P1 cotransduction: 8% <ref name='LIB:Nichols_linked_Tn'/>  
est. P1 cotransduction: 8% <ref name='LIB:Nichols_linked_Tn'/>  


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|- {{table_heading_style}}
|- {{table_heading_style}}
!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-  
|-  
|
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG12099 EcoCyc:EG12099]
|
|-
|
[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
|
[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB2023 EchoBASE:EB2023]
|
|-
|
[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
|
|
[http://chase.ou.edu/macro/searchlink.php?bnum=b4147 EcoliGenExpDB:b4147]
EcoCyc:EG12099
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
[[EcoGene]]
|
|
[http://BioCyc.org/ECOL199310/new-image?object=C5232 ECOL199310CYC:C5232]
EcoGene:EG12099
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://ecogene.org/geneinfo.php?eg_id=EG12099 EcoGene:EG12099]
RegulonDB:ECK120002004
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
|
[http://BioCyc.org/ECOO157/new-image?object=EFP ECOO157CYC:EFP]
[http://www.ncbi.nlm.nih.gov/gene?term=948661 GeneID:948661]
|
|
Escherichia coli str. K-12 substr. MG1655
|-  
|-  
|
|
[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/34470 CGSC:34470]
EchoBASE:EB2023
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
[[ASAP]]
|
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0013583&formSubmitReturn=1 ASAP:ABE-0013583]
ASAP:ABE-0013583
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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==References==
==References==
{{BacktoTop}}
{{BacktoTop}}
 
{{RefHelp}}
<references/>
<references />
__NOTOC__
__NOTOC__
</protect></noinclude>
===Categories===
===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:MG1655]]
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[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:efp genes]]
[[Category:efp genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

Latest revision as of 20:51, 19 September 2013

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

[back to top]


See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

efp

Mnemonic

Elongation Factor-Peptidyltransferase

Synonyms

ECK4141, b4147, JW4107[1], JW4107


Notes

Location(s) and DNA Sequence

[back to top]


See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

94.27 minutes 

MG1655: 4373722..4374288
<gbrowseImage> name=NC_000913:4373722..4374288 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4354410..4354976
<gbrowseImage> name=NC_012967:4354410..4354976 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4312457..4313023
<gbrowseImage> name=NC_012759:4312457..4313023 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4380379..4380945
<gbrowseImage> name=NC_007779:4380379..4380945 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4474084..4474650
<gbrowseImage> name=NC_010473:4474084..4474650 source=DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4373725

Edman degradation

PMID:1956781[2]
PMID:9298646[3]



Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δefp (Keio:JW4107)

deletion

deletion

PMID:16738554[4]

Shigen
CGSC10957[5]

Δefp-772::kan

PMID:16738554[4]

CGSC:100688



Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4107

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCGCAACGTACTATAGCAACGA

Primer 2:CCtTTCACGCGAGAGACGTATTC

9B1

Kohara Phage

Genobase

PMID:3038334[7]

1D4

Kohara Phage

Genobase

PMID:3038334[7]

3H6

Kohara Phage

Genobase

PMID:3038334[7]

cadB2231::Tn10

Linked marker

CAG18488 = CGSC7479[5]

est. P1 cotransduction: 54% [8]
Synonyms:zjd-2231::Tn10

cycA30::Tn10

Linked marker

CAG12073 = CGSC7482[5]

est. P1 cotransduction: 8% [8]


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12099

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12099

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002004

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948661

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2023

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013583

Escherichia coli str. K-12 substr. MG1655


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Aoki, H et al. (1991) Cloning, sequencing and overexpression of the gene for prokaryotic factor EF-P involved in peptide bond synthesis. Nucleic Acids Res. 19 6215-20 PubMed
  3. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  4. ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 7.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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