dppA:Gene: Difference between revisions

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|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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{|{{Prettytable}}
{|   id="U46a9f89aeca07"  class=" tableEdit Gene_nomenclature_table" 
 
|-
!align=left {{table_heading_style}} |Standard name
||
''dppA''
|-
|-
!{{table_heading_style}} |Standard name
!align=left {{table_heading_style}} |Mnemonic
|
||
''dppA''
Dipeptide permease system
|-
|-
!{{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Synonyms
|
||
''ECK3531, b3544, JW3513, alu, dpp, fpp''<ref name='LIB:Riley_2006'/>, fpp''


|-
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!{{table_heading_style}} |Synonyms
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17637.U46a9f89aeca07&page=17637&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table]</span> ||
|
''ECK3531, b3544, JW3513, alu, dpp, fpp''<ref name='LIB:Riley_2006'/>
|-
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===Notes===
===Notes===
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{|class = 'sortable' {{Prettytable}} id='146115'
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="J46a9f89b7a675"  class=" tableEdit Gene_location_table" 
|- {{table_heading_style}}
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!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|-  
|
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MG1655
MG1655
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79.84 minutes&nbsp;
79.84 minutes&nbsp;
|
|
MG1655: 3705728..3704121<br />{{GBrowseFigureByCoord|3703121|3706728|ecoli|MG1655}}
MG1655: 3705728..3704121<br /><gbrowseImage>
name=NC_000913:3704121..3705728
source=MG1655
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10248 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG10248 EcoCyc]
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?q=MG1655:3703121..3706728 GBrowse(MG1655)]
{{GBrowseLink|MG1655|NC_000913|3703121|3706728|GBrowse(MG1655)}}
   
   
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?plugin=FastaDumper&name=MG1655:3704121..3705728&plugin_action=Go FASTA Dump]
{{GBrowseFastaLink|MG1655|NC_000913|3704121|3705728|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG10248 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG10248 EcoCyc Sequence Dump]  
|-
|-
|
[[REL606]]
|
 
|
NC_012967: 3638163..3636556<br /><gbrowseImage>
name=NC_012967:3636556..3638163
source=REL606
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|REL606|NC_012967|3636556|3638163|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|3636556|3638163|FASTA Dump}}
|-
|
[[BW2952]]
|
 
|
NC_012759: 3592615..3594222<br /><gbrowseImage>
name=NC_012759:3592615..3594222
source=BW2952
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|BW2952|NC_012759|3592615|3594222|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|3592615|3594222|FASTA Dump}}
|-  
|
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W3110
W3110
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&nbsp;
&nbsp;
|
|
W3110: 3932710..3934317<br />{{GBrowseFigureByCoord|3931710|3935317|ecoli_W3110|W3110}}  
W3110: 3932710..3934317<br /><gbrowseImage>
name=NC_007779:3932710..3934317
source=W3110
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|W3110|NC_007779|3931710|3935317|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|NC_007779|3932710|3934317|FASTA Dump}}  
|-
|
[[DH10B]]
|
 
|
DH10B: 3803473..3801866<br /><gbrowseImage>
name=NC_010473:3801866..3803473
source=DH10B
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?q=W3110:3931710..3935317 GBrowse(W3110)]
{{GBrowseLink|DH10B|NC_010473|3798652|3806687|GBrowse(DH10B)}}
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?plugin=FastaDumper&name=W3110:3932710..3934317&plugin_action=Go FASTA Dump]
{{GBrowseFastaLink|DH10B|NC_010473|3798652|3806687|FASTA Dump}}
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|[{{SERVER}}{{SCRIPTPATH}}/index.php?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17637.J46a9f89b7a675&page=17637&pagename={{FULLPAGENAMEE}} edit table] || || || ||
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===Notes===
===Notes===
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{|class = 'sortable' {{Prettytable}} id='146116'
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="H46a9f89c3cb55"  class=" tableEdit Gene_sequence_features_table" 
|- {{table_heading_style}}
|- {{table_heading_style}}
!Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
|-class='sortbottom'
|-
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|
Coding Start (SO:0000323)
|
[[MG1655]]
|
3704124
|
Edman degradation
|
PMID:1956284<br />PMID:9298646<br />PMID:9600841<br />PMID:9740056<br />PMID:10972807<br />
|
 
|- class="tableEdit_footer"
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<protect><!--Sequence_notes--></protect>
<protect><!--Sequence_notes--></protect>
====Notes====
====Notes====
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{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" {{Prettytable}}
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;" id="L46a9f89c8611e"  class=" tableEdit Gene_allele_table dataTable" 
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
|-  
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&Delta;dppA (Keio:JW3513)
''dppA(del)'' (Keio:JW3513)
|
|
deletion
deletion
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|
|
[[PMID:16738554]]<ref name=PMID:16738554/>
PMID:16738554
|
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=108880 CGSC10599]<ref name='LIB:cgsc'/>  
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]
 
[http://cgsc2.biology.yale.edu/Strain.php?ID=108880 CGSC10599]<ref name='LIB:cgsc'/>  
|
|
   
   
|-  
|-  
|
|
''&#916;dppA728::kan''
[http://asap.ahabs.wisc.edu/asap/feature_info.php?FeatureID=ABE-0011576 dppA::Tn5KAN-I-SceI] (FB21271)
|
Insertion at nt 837 in Minus orientation
|
 
|
 
|
 
|
PMID:15262929
|
[http://www.genome.wisc.edu/resources/forms/strainrequest.htm E. coli Genome Project:FB21271]
|
does not contain [[pKD46]]
|-
|
''dppA728(del)::kan''
|
|


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|
|
[[PMID:16738554]]<ref name=PMID:16738554/>
PMID:16738554
|
|
[[cgscmutation:100636|CGSC:100636]]
[[cgscmutation:100636|CGSC:100636]]
|
|
   
   
|-
|
''dppA'' in strain PA0113
|
|
|
Resistant to
|
Lysyl-2-aminooxypronate resistance
|
PMID:6389761
|
PA0113
|
|-
|
''dppA::Kan mppA::Cm''
|
|
|
Auxotrophies
|
Abolished heme utilization.
|
PMID:16905647
|
|
See Table 1.


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|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.17637.L46a9f89c8611e&page=17637&pagename={{FULLPAGENAMEE}}&type=&template=Gene_allele_table edit table]</span> || || || || || || ||
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{{Gene_alleles_help}}
 
<protect><!--Alleles_notes--></protect>
<protect><!--Alleles_notes--></protect>
===Notes===
===Notes===


==Genetic Interactions==
{{BacktoTop}}
<protect><!--box uid=bc145ea147f7e36caf09c1bfeed4ee9d.17637.G4e04ba0c1a7dd-->
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="G4e04ba0c1a7dd"  class=" tableEdit Genetic_interaction_table dataTable" 
|- {{table_heading_style}}
!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
|- class="tableEdit_footer"
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===Notes===


==Genetic Resources==
==Genetic Resources==
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!Resource!!Resource Type!!Source!!Notes/Reference
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JW3513
JW3513
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[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
|
[[PMID:16769691]]<ref name=PMID:16769691/>
PMID:16769691


Status:Clone OK
Status:Clone OK
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Primer 2:CCTTCGATAGAGACGTTTTCGAA  
Primer 2:CCTTCGATAGAGACGTTTTCGAA  
|-
|-
|
[[:Category:Kohara_Phage:9F6|9F6]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=602 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:1D1|1D1]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=603 Genobase]
|
PMID:3038334
|-  
|
|
zhg-50::Tn10
zhg-50::Tn10
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CAG18450 = [http://cgsc2.biology.yale.edu/Strain.php?ID=37863 CGSC7450]<ref name='LIB:cgsc'/>
CAG18450 = [http://cgsc2.biology.yale.edu/Strain.php?ID=37863 CGSC7450]<ref name='LIB:cgsc'/>
|
|
est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/>
est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/> <br />
 
Synonyms:zhf-50::Tn10, zhf-5::Tn10  
Synonyms:zhf-50::Tn10, zhf-5::Tn10  
|-
|-  
|
|
zic-4901::Tn10
zic-4901::Tn10
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|
|
est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/>  
est. P1 cotransduction: % <ref name='LIB:Nichols_linked_Tn'/>  
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{{Gene_resources_help}}
 
<protect><!--Gene_resources_notes--></protect>
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===Notes===
===Notes===
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{|class = 'sortable' border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"   
{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="Y46a9f89de629b"  class=" tableEdit Gene_accessions_table"   
|- {{table_heading_style}}
|- {{table_heading_style}}
!|Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-  
|-  
|
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
[[EcoCyc]]
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG10248 EcoCyc:EG10248]
|
|-
|
[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
|
[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB0244 EchoBASE:EB0244]
|
|-
|
[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
|
|
[http://chase.ou.edu/macro/searchlink.php?bnum=b3544 EcoliGenExpDB:b3544]
EcoCyc:EG10248
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
[[EcoGene]]
|
|
[http://BioCyc.org/ECOL199310/new-image?object=C4361 ECOL199310CYC:C4361]
EcoGene:EG10248
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://ecogene.org/geneinfo.php?eg_id=EG10248 EcoGene:EG10248]
RegulonDB:ECK120000242
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
|
[http://BioCyc.org/ECOO157/new-image?object=DPPA ECOO157CYC:DPPA]
[http://www.ncbi.nlm.nih.gov/gene?term=948062 GeneID:948062]
|
|
Escherichia coli str. K-12 substr. MG1655
|-  
|-  
|
|
[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/35111 CGSC:35111]
EchoBASE:EB0244
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-  
|-  
|
|
[https://asap.ahabs.wisc.edu/asap/logon.php ASAP] ([[ASAP|EcoliWikiPage]])
[[ASAP]]
|
|
[http://asap.ahabs.wisc.edu/asap/feature_info.php?LocationID=WIS&FeatureID=0011576&formSubmitReturn=1 ASAP:ABE-0011576] 
ASAP:ABE-0011576
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]


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===Categories===
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[[Category:Gene List:MG1655]]
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[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:dpp genes]]
[[Category:dpp genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

Latest revision as of 20:48, 19 September 2013

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

dppA

Mnemonic

Dipeptide permease system

Synonyms

ECK3531, b3544, JW3513, alu, dpp, fpp[1], fpp

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Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

79.84 minutes 

MG1655: 3705728..3704121
<gbrowseImage> name=NC_000913:3704121..3705728 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 3638163..3636556
<gbrowseImage> name=NC_012967:3636556..3638163 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 3592615..3594222
<gbrowseImage> name=NC_012759:3592615..3594222 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 3932710..3934317
<gbrowseImage> name=NC_007779:3932710..3934317 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 3803473..3801866
<gbrowseImage> name=NC_010473:3801866..3803473 source=DH10B preset=GeneLocation </gbrowseImage>

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Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

3704124

Edman degradation

PMID:1956284[2]
PMID:9298646[3]
PMID:9600841[4]
PMID:9740056[5]
PMID:10972807[6]


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Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

dppA(del) (Keio:JW3513)

deletion

deletion

PMID:16738554[7]

Shigen

CGSC10599[8]

dppA::Tn5KAN-I-SceI (FB21271)

Insertion at nt 837 in Minus orientation

PMID:15262929[9]

E. coli Genome Project:FB21271

does not contain pKD46

dppA728(del)::kan

PMID:16738554[7]

CGSC:100636

dppA in strain PA0113

Resistant to

Lysyl-2-aminooxypronate resistance

PMID:6389761[10]

PA0113

dppA::Kan mppA::Cm

Auxotrophies

Abolished heme utilization.

PMID:16905647[11]

See Table 1.

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Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes
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Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW3513

Plasmid clone

Shigen

PMID:16769691[12]

Status:Clone OK

Primer 1:GCCCGTATTTCCTTGAAAAAGTC

Primer 2:CCTTCGATAGAGACGTTTTCGAA

9F6

Kohara Phage

Genobase

PMID:3038334[13]

1D1

Kohara Phage

Genobase

PMID:3038334[13]

zhg-50::Tn10

Linked marker

CAG18450 = CGSC7450[8]

est. P1 cotransduction: % [14]
Synonyms:zhf-50::Tn10, zhf-5::Tn10

zic-4901::Tn10

Linked marker

CAG18492 = CGSC7457[8]

est. P1 cotransduction: % [14]

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Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG10248

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG10248

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120000242

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948062

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB0244

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0011576

Escherichia coli str. K-12 substr. MG1655

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Notes

Links

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<protect>

References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Abouhamad, WN et al. (1991) Peptide transport and chemotaxis in Escherichia coli and Salmonella typhimurium: characterization of the dipeptide permease (Dpp) and the dipeptide-binding protein. Mol. Microbiol. 5 1035-47 PubMed
  3. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  4. ↑ Wilkins, MR et al. (1998) Protein identification with N and C-terminal sequence tags in proteome projects. J. Mol. Biol. 278 599-608 PubMed
  5. ↑ Tonella, L et al. (1998) '98 Escherichia coli SWISS-2DPAGE database update. Electrophoresis 19 1960-71 PubMed
  6. ↑ Urbanowski, ML et al. (2000) The gcvB gene encodes a small untranslated RNA involved in expression of the dipeptide and oligopeptide transport systems in Escherichia coli. Mol. Microbiol. 37 856-68 PubMed
  7. ↑ 7.0 7.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  8. ↑ 8.0 8.1 8.2 CGSC: The Coli Genetics Stock Center
  9. ↑ Kang, Y et al. (2004) Systematic mutagenesis of the Escherichia coli genome. J. Bacteriol. 186 4921-30 PubMed
  10. ↑ Payne, JW et al. (1984) Transport and hydrolysis of antibacterial peptide analogues in Escherichia coli: backbone-modified aminoxy peptides. J. Gen. Microbiol. 130 2253-65 PubMed
  11. ↑ Létoffé, S et al. (2006) The housekeeping dipeptide permease is the Escherichia coli heme transporter and functions with two optional peptide binding proteins. Proc. Natl. Acad. Sci. U.S.A. 103 12891-6 PubMed
  12. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  13. ↑ 13.0 13.1 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  14. ↑ 14.0 14.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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