efp:Gene: Difference between revisions

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<noinclude>{{TabsTop}}  
<noinclude>{{TabsTop}}  
{{Tab|Quickview|efp}}{{Tab On|Gene|efp}}{{Tab|Gene Product(s)|efp}}{{Tab|Expression|efp}}{{Tab|Evolution|efp}}{{Tab|On One Page|efp}}{{TabsBottom}}
{{Tab|Quickview|efp}}{{Tab On|Gene|efp}}{{Tab|Gene Product(s)|efp}}{{Tab|Expression|efp}}{{Tab|Evolution|efp}}{{Tab|On One Page|efp}}{{TabsBottom}}
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|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Nomenclature |Nomenclature]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Location(s) and DNA Sequence|Location(s) and DNA Sequence]]
|{{Sub}}[[#Sequence Features|Sequence Features]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Alleles and Phenotypes |Alleles and Phenotypes]]
|{{Sub}}[[#Genetic Interactions | Genetic Interactions]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Genetic Resources  |Genetic Resources]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
|{{Sub}}[[#Accessions in Other Databases |Accessions]]
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==Nomenclature==
==Nomenclature==
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{|   id="T46aa084dcf783"  class=" tableEdit Gene_nomenclature_table" 
 
|-
!align=left {{table_heading_style}} |Standard name
||
''efp''
|-
|-
!{{table_heading_style}} |Standard name
!align=left {{table_heading_style}} |Mnemonic
|
||
''efp''
Elongation Factor-Peptidyltransferase
|-
|-
!{{table_heading_style}} |Mnemonic
!align=left {{table_heading_style}} |Synonyms
|
||
''ECK4141, b4147, JW4107''<ref name='LIB:Riley_2006'/>, JW4107''


|-
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!{{table_heading_style}} |Synonyms
|<span class="tableEdit_editLink plainlinks">[{{SERVER}}{{SCRIPTPATH}}?title=Special:TableEdit&id=421aa90e079fa326b6494f812ad13e79.18855.T46aa084dcf783&page=18855&pagename={{FULLPAGENAMEE}}&type=1&template=Gene_nomenclature_table edit table]</span> ||
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''ECK4141, b4147, JW4107''<ref name='LIB:Riley_2006'/>
|-
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<!--Nomenclature_notes--></protect>
<!--Nomenclature_notes--></protect>
===Notes===
===Notes===
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==Location(s) and DNA Sequence==
==Location(s) and DNA Sequence==
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<protect>
<protect>
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="K46aa084e5ed05"  class=" tableEdit Gene_location_table" 
|- {{table_heading_style}}
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!Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
!|Strain!!Map location!!Genome coordinates!!Genome browsers!!Sequence links
|-
|-  
|
|
MG1655
MG1655
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94.27 minutes&nbsp;
94.27 minutes&nbsp;
|
|
MG1655: 4373722..4374288<br />{{GBrowseFigureByCoord|4372722|4375288|ecoli|MG1655}}
MG1655: 4373722..4374288<br /><gbrowseImage>
name=NC_000913:4373722..4374288
source=MG1655
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG12099 EcoCyc]
*[http://biocyc.org/ECOLI/NEW-IMAGE?type=LOCUS-POSITION&chromosome=COLI-K12&object=EG12099 EcoCyc]
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?q=MG1655:4372722..4375288 GBrowse(MG1655)]
{{GBrowseLink|MG1655|NC_000913|4372722|4375288|GBrowse(MG1655)}}
   
   
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli/?plugin=FastaDumper&name=MG1655:4373722..4374288&plugin_action=Go FASTA Dump]
{{GBrowseFastaLink|MG1655|NC_000913|4373722|4374288|FASTA Dump}}
*[http://biocyc.org/ECOLI/sequence?object=EG12099 EcoCyc Sequence Dump]  
*[http://biocyc.org/ECOLI/sequence?object=EG12099 EcoCyc Sequence Dump]  
|-
|-
|
[[REL606]]
|
 
|
NC_012967: 4354410..4354976<br /><gbrowseImage>
name=NC_012967:4354410..4354976
source=REL606
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|REL606|NC_012967|4354410|4354976|GBrowse (REL606)}}
|
{{GBrowseFastaLink|REL606|NC_012967|4354410|4354976|FASTA Dump}}
|-
|
[[BW2952]]
|
 
|
NC_012759: 4312457..4313023<br /><gbrowseImage>
name=NC_012759:4312457..4313023
source=BW2952
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|BW2952|NC_012759|4312457|4313023|GBrowse (BW2952)}}
|
{{GBrowseFastaLink|BW2952|NC_012759|4312457|4313023|FASTA Dump}}
|-  
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|
W3110
W3110
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&nbsp;
&nbsp;
|
|
W3110: 4380379..4380945<br />{{GBrowseFigureByCoord|4379379|4381945|ecoli_W3110|W3110}}  
W3110: 4380379..4380945<br /><gbrowseImage>
name=NC_007779:4380379..4380945
source=W3110
preset=GeneLocation
</gbrowseImage>
 
|
{{GBrowseLink|W3110|NC_007779|4379379|4381945|GBrowse(W3110)}}
|
{{GBrowseFastaLink|W3110|NC_007779|4380379|4380945|FASTA Dump}}  
|-
|
[[DH10B]]
|
 
|
DH10B: 4474084..4474650<br /><gbrowseImage>
name=NC_010473:4474084..4474650
source=DH10B
preset=GeneLocation
</gbrowseImage>
 
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?q=W3110:4379379..4381945 GBrowse(W3110)]
{{GBrowseLink|DH10B|NC_010473|4472952|4475782|GBrowse(DH10B)}}
|
|
*[http://dimer.tamu.edu/cgi-bin/gb/gbrowse/ecoli_W3110/?plugin=FastaDumper&name=W3110:4380379..4380945&plugin_action=Go FASTA Dump]
{{GBrowseFastaLink|DH10B|NC_010473|4472952|4475782|FASTA Dump}}
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===Notes===
===Notes===
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==Sequence Features==
==Sequence Features==
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="K46aa084f26be8"  class=" tableEdit Gene_sequence_features_table" 
|- {{table_heading_style}}
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!Feature type!!Location!!Description
!|Feature Type!!Strain!!Genomic Location!!Evidence!!Reference!!Notes
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Coding Start (SO:0000323)
|
[[MG1655]]
|
4373725
|
Edman degradation
|
PMID:1956781<br />PMID:9298646<br />
|
 
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<protect><!--Sequence_notes--></protect>
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====Notes====
====Notes====
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==Alleles and Phenotypes==
==Alleles and Phenotypes==
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{| border="2" cellpadding="4" cellspacing="0" style="margin: 1em 1em 1em 0; border: 1px #aaa solid; border-collapse: collapse;"  id="D46aa084f72235"  class=" tableEdit Gene_allele_table dataTable" 
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!Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!CGSC Mutation ID #
!|Allele!!Nt change(s)!!AA change(s)!!Phenotype: Type!!Phenotype: Description!!Reference!!Availability!!Comments
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&Delta;efp (Keio:JW4107)
|
deletion
|
deletion
|
 
|
 
|
PMID:16738554
|
[http://www.shigen.nig.ac.jp/ecoli/strain/keioCollectionQueryAction.do Shigen]<br>[http://cgsc2.biology.yale.edu/Strain.php?ID=109238 CGSC10957]<ref name='LIB:cgsc'/>
|
|-
|
''&#916;efp-772::kan''
|
 
|
 
|
 
|
 
|
PMID:16738554
|
[[cgscmutation:100688|CGSC:100688]]
|
 
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<protect><!--Alleles_notes--></protect>
<protect><!--Alleles_notes--></protect>
===Notes===
===Notes===


==Genetic Interactions==
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!|Interactor!!Interaction!!Allele!!Score(s)!!Reference(s)!!Accessions!!Notes
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===Notes===


==Genetic Resources==
==Genetic Resources==
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!Resource!!Resource Type!!Source!!Notes/Reference
!|Resource!!Resource Type!!Source!!Notes/Reference
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JW4107
|
Plasmid clone
|
[http://www.shigen.nig.ac.jp/ecoli/strain/askaCloneQueryAction.do?strainTypeId=9 Shigen]
|
PMID:16769691
 
Status:Clone OK
 
Primer 1:GCCGCAACGTACTATAGCAACGA
 
Primer 2:CCtTTCACGCGAGAGACGTATTC
|-
|
[[:Category:Kohara_Phage:9B1|9B1]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=649 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:1D4|1D4]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=650 Genobase]
|
PMID:3038334
|-
|
[[:Category:Kohara_Phage:3H6|3H6]]
|
Kohara Phage
|
[http://ecoli.aist-nara.ac.jp/GB6/kohara_info.jsp?fword=651 Genobase]
|
PMID:3038334
|-
|
[[cadB:Gene|cadB2231::Tn10]]
|
Linked marker
|
CAG18488 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40596 CGSC7479]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 54% <ref name='LIB:Nichols_linked_Tn'/> <br />
Synonyms:zjd-2231::Tn10
|-
|
[[cycA:Gene|cycA30::Tn10]]
|
Linked marker
|
CAG12073 = [http://cgsc2.biology.yale.edu/Strain.php?ID=40604 CGSC7482]<ref name='LIB:cgsc'/>
|
est. P1 cotransduction: 8% <ref name='LIB:Nichols_linked_Tn'/>
 
|- class="tableEdit_footer"
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===Notes===
===Notes===
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==Accessions in Other Databases==
==Accessions in Other Databases==
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|- {{table_heading_style}}
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!Database!!Accession!!Notes
!|Database!!Accession!!Notes
|-
|-  
|
[http://ecocyc.org EcoCyc] ([[EcoCyc|EcoliWiki Page]])
|
[http://biocyc.org/ECOLI/NEW-IMAGE?type=NIL&object=EG12099 EcoCyc:EG12099] 
|
|-
|
|
[http://www.ecoli-york.org EchoBASE] ([[EchoBASE|EcoliWiki Page]])
[[EcoCyc]]
|
|
[http://www.york.ac.uk/res/thomas/Gene.cfm?recordID=EB2023 EchoBASE:EB2023] 
EcoCyc:EG12099
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://chase.ou.edu/oubcf/index.php EcoliGenExpDB] ([[EcoliGenExpDB|EcoliWiki Page]])
[[EcoGene]]
|
|
[http://chase.ou.edu/macro/searchlink.php?bnum=b4147 EcoliGenExpDB:b4147] 
EcoGene:EG12099
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://BioCyc.org/ECOL199310/ ECOL199310CYC] ([[ECOL199310CYC|EcoliWiki Page]])
[[RegulonDB]]
|
|
[http://BioCyc.org/ECOL199310/new-image?object=C5232 ECOL199310CYC:C5232] 
RegulonDB:ECK120002004
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://ecogene.org EcoGene] ([[EcoGene|EcoliWiki Page]])  
[http://www.ncbi.nlm.nih.gov/gene NCBI] ([[NCBI|EcoliWiki Page]])
|
|
[http://ecogene.org/geneinfo.php?eg_id=EG12099 EcoGene:EG12099]
[http://www.ncbi.nlm.nih.gov/gene?term=948661 GeneID:948661]
|
|
Escherichia coli str. K-12 substr. MG1655
|-
|-  
|
|
[http://BioCyc.org/ECOO157/ ECOO157CYC] ([[ECOO157CYC|EcoliWiki Page]])
[[EchoBASE]]
|
|
[http://BioCyc.org/ECOO157/new-image?object=EFP ECOO157CYC:EFP] 
EchoBASE:EB2023
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
|-
|-  
|
|
[http://cgsc.biology.yale.edu CGSC] ([[CGSC|EcoliWiki Page]])
[[ASAP]]
|
|
[http://cgsc.biology.yale.edu/cgi-bin/sybgw/cgsc/Site/34470 CGSC:34470] 
ASAP:ABE-0013583
|
|
''Escherichia coli'' str. K-12 substr. [[:Category:Strain:MG1655|MG1655]]
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===Notes===
===Notes===
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==Links==
==Links==
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==References==
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===Categories===
[[Category:Gene List:MG1655]]
[[Category:Gene List:W3110]]
[[Category:Genes in the Keio knockout collection]]
[[Category:Genes in the ASKA clone collection]]
[[Category:efp genes]][[Category:Gene_List:DH10B]][[Category:Gene_List:REL606]]
[[Category:Gene_List:BW2952]]

Latest revision as of 20:51, 19 September 2013

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Quickview   Gene   Gene Product(s)   Expression   Evolution   On One Page    
Nomenclature Location(s) and DNA Sequence Sequence Features Alleles and Phenotypes Genetic Interactions Genetic Resources Accessions Links References Suggestions

Nomenclature

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See Help:Gene_nomenclature for help with entering information in the Gene Nomenclature table.

Standard name

efp

Mnemonic

Elongation Factor-Peptidyltransferase

Synonyms

ECK4141, b4147, JW4107[1], JW4107


Notes

Location(s) and DNA Sequence

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See Help:Gene_location for help entering information in the Gene Location and DNA sequence table.

Strain Map location Genome coordinates Genome browsers Sequence links

MG1655

94.27 minutes 

MG1655: 4373722..4374288
<gbrowseImage> name=NC_000913:4373722..4374288 source=MG1655 preset=GeneLocation </gbrowseImage>

REL606

NC_012967: 4354410..4354976
<gbrowseImage> name=NC_012967:4354410..4354976 source=REL606 preset=GeneLocation </gbrowseImage>

BW2952

NC_012759: 4312457..4313023
<gbrowseImage> name=NC_012759:4312457..4313023 source=BW2952 preset=GeneLocation </gbrowseImage>

W3110

 

W3110: 4380379..4380945
<gbrowseImage> name=NC_007779:4380379..4380945 source=W3110 preset=GeneLocation </gbrowseImage>

DH10B

DH10B: 4474084..4474650
<gbrowseImage> name=NC_010473:4474084..4474650 source=DH10B preset=GeneLocation </gbrowseImage>


Notes

Sequence Features

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See Help:Gene_sequence_features for help in entering sequence features in EcoliWiki.

Feature Type Strain Genomic Location Evidence Reference Notes

Coding Start (SO:0000323)

MG1655

4373725

Edman degradation

PMID:1956781[2]
PMID:9298646[3]



Notes

Alleles and Phenotypes

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See Help:Gene_alleles for how to enter or edit alleles and phenotypes in EcoliWiki.

Allele Nt change(s) AA change(s) Phenotype: Type Phenotype: Description Reference Availability Comments

Δefp (Keio:JW4107)

deletion

deletion

PMID:16738554[4]

Shigen
CGSC10957[5]

Δefp-772::kan

PMID:16738554[4]

CGSC:100688



Notes

Genetic Interactions

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Interactor Interaction Allele Score(s) Reference(s) Accessions Notes


Notes

Genetic Resources

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See Help:Gene_resources for help entering information into the Genetic Resources table.

Resource Resource Type Source Notes/Reference

JW4107

Plasmid clone

Shigen

PMID:16769691[6]

Status:Clone OK

Primer 1:GCCGCAACGTACTATAGCAACGA

Primer 2:CCtTTCACGCGAGAGACGTATTC

9B1

Kohara Phage

Genobase

PMID:3038334[7]

1D4

Kohara Phage

Genobase

PMID:3038334[7]

3H6

Kohara Phage

Genobase

PMID:3038334[7]

cadB2231::Tn10

Linked marker

CAG18488 = CGSC7479[5]

est. P1 cotransduction: 54% [8]
Synonyms:zjd-2231::Tn10

cycA30::Tn10

Linked marker

CAG12073 = CGSC7482[5]

est. P1 cotransduction: 8% [8]


Notes

Accessions in Other Databases

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See Help:Gene_accessions for help with entering information into the Gene Accessions table.

Database Accession Notes

EcoCyc

EcoCyc:EG12099

Escherichia coli str. K-12 substr. MG1655

EcoGene

EcoGene:EG12099

Escherichia coli str. K-12 substr. MG1655

RegulonDB

RegulonDB:ECK120002004

Escherichia coli str. K-12 substr. MG1655

NCBI (EcoliWiki Page)

GeneID:948661

Escherichia coli str. K-12 substr. MG1655

EchoBASE

EchoBASE:EB2023

Escherichia coli str. K-12 substr. MG1655

ASAP

ASAP:ABE-0013583

Escherichia coli str. K-12 substr. MG1655


Notes

Links

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References

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See Help:References for how to manage references in EcoliWiki.

  1. ↑ Riley, M. et al. (2006) Nucleic Acids Res 34:1-6 (corrected supplemental data from B. Wanner)
  2. ↑ Aoki, H et al. (1991) Cloning, sequencing and overexpression of the gene for prokaryotic factor EF-P involved in peptide bond synthesis. Nucleic Acids Res. 19 6215-20 PubMed
  3. ↑ Link, AJ et al. (1997) Comparing the predicted and observed properties of proteins encoded in the genome of Escherichia coli K-12. Electrophoresis 18 1259-313 PubMed
  4. ↑ 4.0 4.1 Baba, T et al. (2006) Construction of Escherichia coli K-12 in-frame, single-gene knockout mutants: the Keio collection. Mol. Syst. Biol. 2 2006.0008 PubMed
  5. ↑ 5.0 5.1 5.2 CGSC: The Coli Genetics Stock Center
  6. ↑ Kitagawa, M et al. (2005) Complete set of ORF clones of Escherichia coli ASKA library (a complete set of E. coli K-12 ORF archive): unique resources for biological research. DNA Res. 12 291-9 PubMed
  7. ↑ 7.0 7.1 7.2 Kohara, Y et al. (1987) The physical map of the whole E. coli chromosome: application of a new strategy for rapid analysis and sorting of a large genomic library. Cell 50 495-508 PubMed
  8. ↑ 8.0 8.1 The Tn10 insertion sites determined by Nichols et al. 1998 (PMID:9829956) were reannotated by alignment with E. coli K-12 genome sequence (GenBank accession NC_000913). P1 contransduction frequencies were calculated using the formula of Wu (PMID:5338813).

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